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	Mir-223-3p functions as a tumor suppressor in lung squamous cell carcinoma by mir-223-3p-mutant p53 regulatory feedback loop
	Luo, Peng1; Wang, Qi2; Ye, Yuanyuan3; Zhang, Ju1; Lu, Dapeng1; Cheng, Longqiang2; Zhou, Hangcheng4; Xie, Mingran5; Wang, Baolong6
刊名	Journal of experimental & clinical cancer research
	2019-02-12
卷号	38页码:12
关键词	Mutant p53-mir-223-3p-feedback loop-lung squamous cell carcinoma
ISSN号	1756-9966
DOI	10.1186/s13046-019-1079-1
通讯作者	Wang, baolong(wbl196555@163.com)
英文摘要	Background: micrornas have an important role in diverse biological processes including tumorigenesis. mir-223 has been reported to be deregulated in several human cancer types. however, its biological role has not been functionally characterized in lung squamous cell carcinoma (lscc). the following study investigates the role of mir-223-3p in lscc growth and metastasis and its underlying mechanism. methods: microrna profiling analyses were conducted to determine differential mirnas expression levels in lscc tumor tissues that successfully formed xenografts in immunocompromised mice (xg) and failed tumor tissues (no-xg). rt-pcr and in situ hybridization (ish) was performed to evaluate the expression of mir-223-3p in 12 paired adjacent normal tissues and lscc specimens. cell proliferation and migration were assessed by cck-8, colony formation and transwell assay, respectively. the role of mir-223-3p in lscc tumorigenesis was examined using xenograft nude models. bioinformatics analysis, dual-luciferase reporter assays, chromatin immunoprecipitation (chip) assay and western blot analysis were used to identify the direct target of mir-223-3p and its interactions. results: mir-223-3p was downregulated in lscc tissues that successfully formed xenografts (xg) compared with tumor tissues that failed (no-xg), which was also significantly reduced in lscc tissues compared with the adjacent normal tissues. gain- and loss-of function experiments showed that mir-223-3p inhibited proliferation and migration in vitro. more importantly, mir-223-3p overexpression greatly suppressed tumor growth in vivo. mechanistically, we found that mutant p53 bound to the promoter region of mir-223 and reduced its transcription. meanwhile, p53 is a direct target of mir-223-3p. thus, mir-223-3p regulated mutant p53 expression in a feedback loop that inhibited cell proliferation and migration. conclusions: our study identified mir-223-3p, as a tumor suppressor gene, markedly inhibited cell proliferation and migration via mir-223-3p-mutant p53 feedback loop, which suggested mir-223-3p might be a new therapeutic target in lscc bearing p53 mutations.
WOS关键词	GAIN-OF-FUNCTION ; MUTANT P53 ; CANCER ; XENOGRAFTS ; EXPRESSION ; DIAGNOSIS ; GENES ; AXIS
WOS研究方向	Oncology
WOS类目	Oncology
语种	英语
出版者	BMC
WOS记录号	WOS:000458776500003
内容类型	期刊论文
URI标识	http://www.corc.org.cn/handle/1471x/2372744
专题	大连化学物理研究所
通讯作者	Wang, Baolong
作者单位	1.Univ Sci & Technol China, Affiliated Hosp 1, Dept Clin Lab, Hefei, Anhui, Peoples R China 2.Anhui Med Univ, Hefei, Anhui, Peoples R China 3.Univ Sci & Technol China, Sch Life Sci, Hefei, Anhui, Peoples R China 4.Univ Sci & Technol China, Affiliated Hosp 1, Dept Pathol, Hefei, Anhui, Peoples R China 5.Univ Sci & Technol China, Affiliated Hosp 1, Dept Thorac Surg, Hefei, Anhui, Peoples R China 6.Univ Sci & Technol China, Affiliated Hosp USTC 1, Dept Clin Lab, Div Life Sci & Med, Hefei 230001, Anhui, Peoples R China
推荐引用方式 GB/T 7714	Luo, Peng,Wang, Qi,Ye, Yuanyuan,et al. Mir-223-3p functions as a tumor suppressor in lung squamous cell carcinoma by mir-223-3p-mutant p53 regulatory feedback loop[J]. Journal of experimental & clinical cancer research,2019,38:12.
APA	Luo, Peng.,Wang, Qi.,Ye, Yuanyuan.,Zhang, Ju.,Lu, Dapeng.,...&Wang, Baolong.(2019).Mir-223-3p functions as a tumor suppressor in lung squamous cell carcinoma by mir-223-3p-mutant p53 regulatory feedback loop.Journal of experimental & clinical cancer research,38,12.
MLA	Luo, Peng,et al."Mir-223-3p functions as a tumor suppressor in lung squamous cell carcinoma by mir-223-3p-mutant p53 regulatory feedback loop".Journal of experimental & clinical cancer research 38(2019):12.

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