An efficient methodology for cryopreservation of spermatozoa of red seabream, Pagrus major, with 2-mL cryovials

	An efficient methodology for cryopreservation of spermatozoa of red seabream, Pagrus major, with 2-mL cryovials
	Liu, Qinghua ; Li, Jun ; Zhang, Shicui ; Ding, Fuhong ; Xu, Xizhang ; Xiao, Zhizhong ; Xu, Shihong
刊名	JOURNAL OF THE WORLD AQUACULTURE SOCIETY
	2006-09-01
卷号	37 期号:3 页码:289-297
关键词	Turbot Scophthalmus-maximus Large-scale Fertilization Cryogenic Preservation Sperm Cryopreservation Clarias-gariepinus African Catfish Cryoprotectants Carp Motility Straws
ISSN号	0893-8849
文献子类	Article
英文摘要	The aim of this study was to optimize the cryopreservation protocols for the sperm of red seabream, Pagrus major. The 2-mL cryovials and programmable freezer were employed for cryopreservation. Six extenders, six cryoprotectants in various concentrations ranging from 6 to 20% (v/v), four cooling rates, and three thawing temperatures were evaluated by postthaw sperm motility and fertility. The ratio of sperm to egg for postthaw sperm fertilization trials was experimentally standardized and was optimal at 500:1. The best motility of postthaw sperm (79.4 +/- 4.7% to 88.6 +/- 8.0%), fertilization rates (89.6 +/- 2.9 to 95.6 +/- 1.9%), and hatching rates (85.3 +/- 5.1% to 91.4 +/- 4.3%) were achieved when Cortland extender, dimethyl sulfoxide (15, 18, and 20%) or ethylene glycol (9, 12%) as cryoprotectants, 20 C/min as the cooling rate, and 40 C as the thawing temperature were employed. Moreover, the results on embryonic development were not significantly different between cryopreserved sperm and fresh sperm during incubation process. In conclusion, these methods of cryopreservation of red seabream sperm are suitable for routine aquaculture application and preservation of genetic resources.; The aim of this study was to optimize the cryopreservation protocols for the sperm of red seabream, Pagrus major. The 2-mL cryovials and programmable freezer were employed for cryopreservation. Six extenders, six cryoprotectants in various concentrations ranging from 6 to 20% (v/v), four cooling rates, and three thawing temperatures were evaluated by postthaw sperm motility and fertility. The ratio of sperm to egg for postthaw sperm fertilization trials was experimentally standardized and was optimal at 500:1. The best motility of postthaw sperm (79.4 +/- 4.7% to 88.6 +/- 8.0%), fertilization rates (89.6 +/- 2.9 to 95.6 +/- 1.9%), and hatching rates (85.3 +/- 5.1% to 91.4 +/- 4.3%) were achieved when Cortland extender, dimethyl sulfoxide (15, 18, and 20%) or ethylene glycol (9, 12%) as cryoprotectants, 20 C/min as the cooling rate, and 40 C as the thawing temperature were employed. Moreover, the results on embryonic development were not significantly different between cryopreserved sperm and fresh sperm during incubation process. In conclusion, these methods of cryopreservation of red seabream sperm are suitable for routine aquaculture application and preservation of genetic resources.
学科主题	Fisheries
语种	英语
WOS记录号	WOS:000239617000006
公开日期	2010-12-24
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/6149]
专题	海洋研究所_海洋生物技术研发中心
作者单位	1.Chinese Acad Sci, Inst Oceanol, Ctr Biotechnol, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100031, Peoples R China 3.Chinese Acad Sci, Inst Oceanol, Ctr Biotechnol R&D, Qingdao 266071, Peoples R China
推荐引用方式 GB/T 7714	Liu, Qinghua,Li, Jun,Zhang, Shicui,et al. An efficient methodology for cryopreservation of spermatozoa of red seabream, Pagrus major, with 2-mL cryovials[J]. JOURNAL OF THE WORLD AQUACULTURE SOCIETY,2006,37(3):289-297.
APA	Liu, Qinghua.,Li, Jun.,Zhang, Shicui.,Ding, Fuhong.,Xu, Xizhang.,...&Xu, Shihong.(2006).An efficient methodology for cryopreservation of spermatozoa of red seabream, Pagrus major, with 2-mL cryovials.JOURNAL OF THE WORLD AQUACULTURE SOCIETY,37(3),289-297.
MLA	Liu, Qinghua,et al."An efficient methodology for cryopreservation of spermatozoa of red seabream, Pagrus major, with 2-mL cryovials".JOURNAL OF THE WORLD AQUACULTURE SOCIETY 37.3(2006):289-297.