Chromosomal mapping of major ribosomal rRNA genes in the hard clam (Mercenaria mercenaria) using fluorescence in situ hybridization

doi:10.1007/s00227-006-0453-7

	Chromosomal mapping of major ribosomal rRNA genes in the hard clam (Mercenaria mercenaria) using fluorescence in situ hybridization
	Wang, Yongping ; Guo, Ximing
刊名	MARINE BIOLOGY
	2007-03-01
卷号	150 期号:6 页码:1183-1189
关键词	Crassostrea-virginica Bivalve Mollusks Donax-trunculus Pectinidae Karyotype Location Sequence Pacific Oyster Gigas
ISSN号	0025-3162
DOI	10.1007/s00227-006-0453-7
文献子类	Article
英文摘要	Karyotype and chromosomal location of the major ribosomal RNA genes were studied in the hard clam (Mercenaria mercenaria Linnaeus) using fluorescence in situ hybridization (FISH). Metaphase chromosomes were obtained from early embryos. Internal transcribed spacers (ITS) between major RNA genes were amplified and used as FISH probes. The probes were labeled with digoxigenin-11-dUTP by polymerase chain reaction and detected with fluorescein-labeled anti-digoxigenin antibodies. FISH with the ITS probes produced two to four signals per nucleus or metaphase. M. mercenaria had a haploid number of 19 chromosomes with a karyotype of seven metacentric, four metacentric or submetacentric, seven submetacentric, and one submetacentric or subtelocentric chromosomes (7M + 4M/SM + 7SM + 1SM/ST). Two ITS loci were observed: one located near the centromere on the long arm of Chromosome 10 and the other at the telomere of the short arm of Chromosome 12. FISH signals on Chromosome 10 are strong and consistent, while signals on Chromosome 12 are variable. This study provides the first karyotype and chromosomal assignment of the major RNA genes in M. mercenaria. Similar studies in a wide range of species are needed to understand the role of chromosomal changes in bivalve evolution.; Karyotype and chromosomal location of the major ribosomal RNA genes were studied in the hard clam (Mercenaria mercenaria Linnaeus) using fluorescence in situ hybridization (FISH). Metaphase chromosomes were obtained from early embryos. Internal transcribed spacers (ITS) between major RNA genes were amplified and used as FISH probes. The probes were labeled with digoxigenin-11-dUTP by polymerase chain reaction and detected with fluorescein-labeled anti-digoxigenin antibodies. FISH with the ITS probes produced two to four signals per nucleus or metaphase. M. mercenaria had a haploid number of 19 chromosomes with a karyotype of seven metacentric, four metacentric or submetacentric, seven submetacentric, and one submetacentric or subtelocentric chromosomes (7M + 4M/SM + 7SM + 1SM/ST). Two ITS loci were observed: one located near the centromere on the long arm of Chromosome 10 and the other at the telomere of the short arm of Chromosome 12. FISH signals on Chromosome 10 are strong and consistent, while signals on Chromosome 12 are variable. This study provides the first karyotype and chromosomal assignment of the major RNA genes in M. mercenaria. Similar studies in a wide range of species are needed to understand the role of chromosomal changes in bivalve evolution.
学科主题	Marine & Freshwater Biology
URL标识	查看原文
语种	英语
WOS记录号	WOS:000244454900014
公开日期	2010-12-24
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/6103]
专题	海洋研究所_海洋生物技术研发中心
作者单位	1.Rutgers State Univ, Inst Marine & Coastal Sci, Haskin Shellfish Res Lab, Port Norris, NJ 08349 USA 2.Chinese Acad Sci, Inst Oceanol, Expt Marine Biol Lab, Qingdao 266071, Shandong, Peoples R China
推荐引用方式 GB/T 7714	Wang, Yongping,Guo, Ximing. Chromosomal mapping of major ribosomal rRNA genes in the hard clam (Mercenaria mercenaria) using fluorescence in situ hybridization[J]. MARINE BIOLOGY,2007,150(6):1183-1189.
APA	Wang, Yongping,&Guo, Ximing.(2007).Chromosomal mapping of major ribosomal rRNA genes in the hard clam (Mercenaria mercenaria) using fluorescence in situ hybridization.MARINE BIOLOGY,150(6),1183-1189.
MLA	Wang, Yongping,et al."Chromosomal mapping of major ribosomal rRNA genes in the hard clam (Mercenaria mercenaria) using fluorescence in situ hybridization".MARINE BIOLOGY 150.6(2007):1183-1189.