Simultaneous enumeration of diatom, protozoa and meiobenthos from marine sediments using Ludox-QPS method

doi:10.1007/s00343-009-9224-x

CORC > 海洋研究所 > 中国科学院海洋研究所 > 海洋生物分类与系统演化实验室

	Simultaneous enumeration of diatom, protozoa and meiobenthos from marine sediments using Ludox-QPS method
	Du Yongfen 1,2; Xu Kuidong1 ; Lei Yanli1
刊名	CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY
	2009-11-01
卷号	27 期号:4 页码:775-783
关键词	Enumeration Extraction Meiobenthos Microbenthos Ludox Hs 40 Centrifugation Quantitative Protargol Stain
ISSN号	0254-4059
DOI	10.1007/s00343-009-9224-x
文献子类	Article
英文摘要	The Ludox-QPS method is a newly developed technique, which combines the Ludox HS 40 density centrifugation and quantitative protargol stain, to enumerate marine ciliates with good taxonomic resolution. We tested the method for simultaneous enumeration of diatoms, protozoa and meiobenthos and compared its extraction efficiency for meiobenthos with that of the routine Ludox-TM centrifugation and a modified protocol using Ludox HS 40. We conducted the evaluation with a sample size of 8.3 ml each from sandy, muddy-sand and muddy sediments collected from the intertidal area of the Yellow Sea in summer 2006 and spring 2007. The Ludox-QPS method not only produced high extraction efficiencies of 97 +/- 1.3% for diatoms and 97.6 +/- 0.8% for ciliates, indicating a reliable enumeration for eukaryotic microbenthos, but also produced excellent extraction efficiencies of on average 97.3% for total meiobenthos, 97.9% for nematodes and 97.8% for copepods from sands, muddy sands and mud. By contrast, the routine Ludox-TM centrifugation obtained only about 74% of total meiobenthos abundance with one extraction cycle, and the modified Ludox HS 40 centrifugation yielded on average 93% of total meiobenthos: 89.4 +/- 2.0% from sands, 93 +/- 4.1% from muddy sands and 97.1 +/- 3.0% from mud. Apart from the sediment type, sample volume was another important factor affecting the extraction efficiency for meiobenthos. The extraction rate was increased to about 96.4% when using the same modified Ludox centrifugation for a 4 ml sediment sample. Besides the excellent extraction efficiency, the Ludox-QPS method obtained higher abundances of meiobenthos, in particular nematodes, than the routine Ludox centrifugation, which frequently resulted in an uncertain loss of small meiobenthos during the sieving process. Statistical analyses demonstrated that there were no significant differences between the meiobenthos communities revealed by the Ludox-QPS method and the modified Ludox HS 40 centrifugation, showing the high efficiency of the Ludox-QPS method for simultaneous enumeration of diatom, protozoa and meiobenthos. Moreover, the comparatively high taxonomic resolution of the method, especially for diatoms and ciliates, makes it feasible to investigate microbial ecology at community level.; The Ludox-QPS method is a newly developed technique, which combines the Ludox HS 40 density centrifugation and quantitative protargol stain, to enumerate marine ciliates with good taxonomic resolution. We tested the method for simultaneous enumeration of diatoms, protozoa and meiobenthos and compared its extraction efficiency for meiobenthos with that of the routine Ludox-TM centrifugation and a modified protocol using Ludox HS 40. We conducted the evaluation with a sample size of 8.3 ml each from sandy, muddy-sand and muddy sediments collected from the intertidal area of the Yellow Sea in summer 2006 and spring 2007. The Ludox-QPS method not only produced high extraction efficiencies of 97 +/- 1.3% for diatoms and 97.6 +/- 0.8% for ciliates, indicating a reliable enumeration for eukaryotic microbenthos, but also produced excellent extraction efficiencies of on average 97.3% for total meiobenthos, 97.9% for nematodes and 97.8% for copepods from sands, muddy sands and mud. By contrast, the routine Ludox-TM centrifugation obtained only about 74% of total meiobenthos abundance with one extraction cycle, and the modified Ludox HS 40 centrifugation yielded on average 93% of total meiobenthos: 89.4 +/- 2.0% from sands, 93 +/- 4.1% from muddy sands and 97.1 +/- 3.0% from mud. Apart from the sediment type, sample volume was another important factor affecting the extraction efficiency for meiobenthos. The extraction rate was increased to about 96.4% when using the same modified Ludox centrifugation for a 4 ml sediment sample. Besides the excellent extraction efficiency, the Ludox-QPS method obtained higher abundances of meiobenthos, in particular nematodes, than the routine Ludox centrifugation, which frequently resulted in an uncertain loss of small meiobenthos during the sieving process. Statistical analyses demonstrated that there were no significant differences between the meiobenthos communities revealed by the Ludox-QPS method and the modified Ludox HS 40 centrifugation, showing the high efficiency of the Ludox-QPS method for simultaneous enumeration of diatom, protozoa and meiobenthos. Moreover, the comparatively high taxonomic resolution of the method, especially for diatoms and ciliates, makes it feasible to investigate microbial ecology at community level.
学科主题	Limnology ; Oceanography
URL标识	查看原文
语种	英语
WOS记录号	WOS:000272422300012
公开日期	2010-12-22
内容类型	期刊论文
版本	出版稿
源URL	[http://ir.qdio.ac.cn/handle/337002/3417]
专题	海洋研究所_海洋生物分类与系统演化实验室
作者单位	1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
推荐引用方式 GB/T 7714	Du Yongfen,Xu Kuidong,Lei Yanli. Simultaneous enumeration of diatom, protozoa and meiobenthos from marine sediments using Ludox-QPS method[J]. CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY,2009,27(4):775-783.
APA	Du Yongfen,Xu Kuidong,&Lei Yanli.(2009).Simultaneous enumeration of diatom, protozoa and meiobenthos from marine sediments using Ludox-QPS method.CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY,27(4),775-783.
MLA	Du Yongfen,et al."Simultaneous enumeration of diatom, protozoa and meiobenthos from marine sediments using Ludox-QPS method".CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY 27.4(2009):775-783.