Sensitive and Rapid Detection of Genetic Modified Soybean (Roundup Ready) by Loop-Mediated Isothermal Amplification

doi:10.1271/bbb.80723

	Sensitive and Rapid Detection of Genetic Modified Soybean (Roundup Ready) by Loop-Mediated Isothermal Amplification
	Liu, Mei 1; Luo, Yan 2; Tao, Ran 1,3; He, Ru 4; Jiang, Keyong 1; Wang, Baojie 1; Wang, Lei 1; Wang, L, Chinese Acad Sci, R&D Ctr Marine Biotechnol, Inst Oceanol, Qingdao 266071, Peoples R China
刊名	BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
	2009-11-01
卷号	73 期号:11 页码:2365-2369
关键词	Loop-mediated Isothermal Amplification Roundup Ready (Rr) Soybean Genetically Modified Organism (Gmo) Detection
ISSN号	0916-8451
DOI	10.1271/bbb.80723
文献子类	Article
英文摘要	Using the LAMP method, a highly specific and sensitive detection system for genetically modified soybean (Roundup Ready) was designed. In this detection system, a set of four primers was designed by targeting the exogenous 35S epsps gene. Target DNA was amplified and visualized on agarose gel within 45 min under isothermal conditions at 65 degrees C. Without gel electrophoresis, the LAMP amplicon was visualized directly in the reaction tube by the addition of SYBR Green I for naked-eye inspection. The detection sensitivity of LAMP was 10-fold higher than the nested PCR established in our laboratory. Moreover, the LAMP method was much quicker, taking only 70 min, as compared with 300 min for nested PCR to complete the analysis of the GM soybean. Compared with traditional PCR approaches, the LAMP procedure is faster and more sensitive, and there is no need for a special PCR machine or electrophoresis equipment. Hence, this method can be a very useful tool for GMO detection and is particularly convenient for fast screening.; Using the LAMP method, a highly specific and sensitive detection system for genetically modified soybean (Roundup Ready) was designed. In this detection system, a set of four primers was designed by targeting the exogenous 35S epsps gene. Target DNA was amplified and visualized on agarose gel within 45 min under isothermal conditions at 65 degrees C. Without gel electrophoresis, the LAMP amplicon was visualized directly in the reaction tube by the addition of SYBR Green I for naked-eye inspection. The detection sensitivity of LAMP was 10-fold higher than the nested PCR established in our laboratory. Moreover, the LAMP method was much quicker, taking only 70 min, as compared with 300 min for nested PCR to complete the analysis of the GM soybean. Compared with traditional PCR approaches, the LAMP procedure is faster and more sensitive, and there is no need for a special PCR machine or electrophoresis equipment. Hence, this method can be a very useful tool for GMO detection and is particularly convenient for fast screening.
学科主题	Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology ; Chemistry, Applied ; Food Science & Technology
URL标识	查看原文
语种	英语
WOS记录号	WOS:000272319900001
公开日期	2010-11-18
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/1855]
专题	海洋研究所_海洋生物技术研发中心
通讯作者	Wang, L, Chinese Acad Sci, R&D Ctr Marine Biotechnol, Inst Oceanol, Qingdao 266071, Peoples R China
作者单位	1.Chinese Acad Sci, R&D Ctr Marine Biotechnol, Inst Oceanol, Qingdao 266071, Peoples R China 2.Ocean Univ China, Sch Life Sci, Qingdao 266003, Peoples R China 3.Rongcheng Entry Exit Inspect & Quarantine Bur, Rongcheng 264300, Peoples R China 4.Haerbin Univ Ind, Weihai Branch, Weihai 264209, Peoples R China
推荐引用方式 GB/T 7714	Liu, Mei,Luo, Yan,Tao, Ran,et al. Sensitive and Rapid Detection of Genetic Modified Soybean (Roundup Ready) by Loop-Mediated Isothermal Amplification[J]. BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY,2009,73(11):2365-2369.
APA	Liu, Mei.,Luo, Yan.,Tao, Ran.,He, Ru.,Jiang, Keyong.,...&Wang, L, Chinese Acad Sci, R&D Ctr Marine Biotechnol, Inst Oceanol, Qingdao 266071, Peoples R China.(2009).Sensitive and Rapid Detection of Genetic Modified Soybean (Roundup Ready) by Loop-Mediated Isothermal Amplification.BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY,73(11),2365-2369.
MLA	Liu, Mei,et al."Sensitive and Rapid Detection of Genetic Modified Soybean (Roundup Ready) by Loop-Mediated Isothermal Amplification".BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY 73.11(2009):2365-2369.