Analysis of the early development in first and backcross generations between Paralichthys olivaceus and Paralichthys dentatus

doi:10.5897/AJB11.581

	Analysis of the early development in first and backcross generations between Paralichthys olivaceus and Paralichthys dentatus
	Sui, Juan 2; Liu, Qinghua 1; He, Tao 1,2; Xiao, Zhizhong 1; Xu, Shihong 1; Ma, Daoyuan 1; Xiao, Yongshuang 1; Lin, Fan 1,2; Li, Jun 1
刊名	AFRICAN JOURNAL OF BIOTECHNOLOGY
	2011-11-02
卷号	10 期号:68 页码:15438-15443
关键词	Paralichthys Olivaceus Paralichthys Dentatus Early Development Inter-specific Hybridization Backcross
ISSN号	1684-5315
DOI	10.5897/AJB11.581
文献子类	Article
英文摘要	This study investigated the performance difference of reciprocal hybrids and backcrosses between Paralichthys olivaceus and Paralichthys dentatus. The fertilization and hatching rates, combined fitness measure, early developmental characteristics and chromosome number were analyzed. The crosses of P. olivaceus female x P. dentatus male (F1), F1 female x P. olivaceus male and F1 female x P. dentatus male could normally fertilize (the fertilization rate: 93.2 to 97.7%), hatch (the hatching rate: 84.4 to 93.1%) and develop during pre-larvae stage. However, the fertilization rate (41.7%) and combined fitness measure (0.327) of P. dentatus female x P. olivaceus male was significantly reduced compared with other crosses. And the embryos of P. dentatus female x P. olivaceus male hatched out with flexural spine and poor vitality and died within 3 days post hatching. In addition, the result of cytogenetics analysis showed two chromosomes were missing in P. dentatus female x P. olivaceus male, which provided evidence of postzygotic barriers in the two species. The results would be useful for better understanding the genetic phenomena of the distant hybridization in fish species.; This study investigated the performance difference of reciprocal hybrids and backcrosses between Paralichthys olivaceus and Paralichthys dentatus. The fertilization and hatching rates, combined fitness measure, early developmental characteristics and chromosome number were analyzed. The crosses of P. olivaceus female x P. dentatus male (F1), F1 female x P. olivaceus male and F1 female x P. dentatus male could normally fertilize (the fertilization rate: 93.2 to 97.7%), hatch (the hatching rate: 84.4 to 93.1%) and develop during pre-larvae stage. However, the fertilization rate (41.7%) and combined fitness measure (0.327) of P. dentatus female x P. olivaceus male was significantly reduced compared with other crosses. And the embryos of P. dentatus female x P. olivaceus male hatched out with flexural spine and poor vitality and died within 3 days post hatching. In addition, the result of cytogenetics analysis showed two chromosomes were missing in P. dentatus female x P. olivaceus male, which provided evidence of postzygotic barriers in the two species. The results would be useful for better understanding the genetic phenomena of the distant hybridization in fish species.
学科主题	Biotechnology & Applied Microbiology
URL标识	查看原文
语种	英语
WOS记录号	WOS:000298551400028
公开日期	2012-07-03
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/11996]
专题	海洋研究所_海洋生物技术研发中心
作者单位	1.Chinese Acad Sci, Inst Oceanol, Ctr Biotechnol R&D, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Sui, Juan,Liu, Qinghua,He, Tao,et al. Analysis of the early development in first and backcross generations between Paralichthys olivaceus and Paralichthys dentatus[J]. AFRICAN JOURNAL OF BIOTECHNOLOGY,2011,10(68):15438-15443.
APA	Sui, Juan.,Liu, Qinghua.,He, Tao.,Xiao, Zhizhong.,Xu, Shihong.,...&Li, Jun.(2011).Analysis of the early development in first and backcross generations between Paralichthys olivaceus and Paralichthys dentatus.AFRICAN JOURNAL OF BIOTECHNOLOGY,10(68),15438-15443.
MLA	Sui, Juan,et al."Analysis of the early development in first and backcross generations between Paralichthys olivaceus and Paralichthys dentatus".AFRICAN JOURNAL OF BIOTECHNOLOGY 10.68(2011):15438-15443.