Identification and assessing the cultivars of Laminaria Lamx. (phaeophyceae) with molecular markers

CORC > 海洋研究所 > 中国科学院海洋研究所 > 海洋环流与波动重点实验室

	Identification and assessing the cultivars of Laminaria Lamx. (phaeophyceae) with molecular markers
	Shi, CJ ; Hu, ZM ; He, YJ ; Zou, YP ; Zhang, DM ; Duan, DL
刊名	JOURNAL OF INTEGRATIVE PLANT BIOLOGY
	2005-03-01
卷号	47 期号:3 页码:283-291
关键词	Genetic Variation Internal Transcribed Spacers (Its) Laminaria Lamx. Phylogenesis Random Amplified Polymorphic Dna (Rapd)
ISSN号	1672-9072
文献子类	Article
英文摘要	Molecular markers were used to identify and assess cultivars of Laminaria Lamx. and to delineate their phylogenetic relationships. Random amplified polymorphic DNA (RAPD) analysis was used for detection. After screening, 11 primers were selected and they yielded 133 bands in all, of which approximately 99.2% were polymorphic. The genetic distances between gametophytes ranged from 0.412 to 0.956. Two clusters were formed with the unweighted pair group method with arithmetic mean (UPGMA) dendrogram based on the simple matching coefficient. All cultivars of Laminaria japonica Aresch. used for breeding in China fell into one cluster. L. japonica from Japan, L. saccharina (L.) Lam., and L. angustata Kjellm. formed the other cluster and showed higher genetic variation than L. japonica from China. Nuclear ribosomal DNA (rDNA) sequences, including internal transcribed spacers (ITS1 and ITS2) were studied and aligned. The nucleotides of the sequences ranged from 634 to 668, with a total of 692 positions including TTS1, ITS2, and the 5.8S coding region. The phylogenetic tree obtained by the neighbor-joining method favored, to some extent, the results revealed by RAPD analysis. The present study indicates that RAPD and ITS analyses could be used to identify and assess Laminaria germplasm and to distinguish some species and, even intraspecies, in Laminaria.; Molecular markers were used to identify and assess cultivars of Laminaria Lamx. and to delineate their phylogenetic relationships. Random amplified polymorphic DNA (RAPD) analysis was used for detection. After screening, 11 primers were selected and they yielded 133 bands in all, of which approximately 99.2% were polymorphic. The genetic distances between gametophytes ranged from 0.412 to 0.956. Two clusters were formed with the unweighted pair group method with arithmetic mean (UPGMA) dendrogram based on the simple matching coefficient. All cultivars of Laminaria japonica Aresch. used for breeding in China fell into one cluster. L. japonica from Japan, L. saccharina (L.) Lam., and L. angustata Kjellm. formed the other cluster and showed higher genetic variation than L. japonica from China. Nuclear ribosomal DNA (rDNA) sequences, including internal transcribed spacers (ITS1 and ITS2) were studied and aligned. The nucleotides of the sequences ranged from 634 to 668, with a total of 692 positions including TTS1, ITS2, and the 5.8S coding region. The phylogenetic tree obtained by the neighbor-joining method favored, to some extent, the results revealed by RAPD analysis. The present study indicates that RAPD and ITS analyses could be used to identify and assess Laminaria germplasm and to distinguish some species and, even intraspecies, in Laminaria.
学科主题	Biochemistry & Molecular Biology ; Plant Sciences
语种	英语
WOS记录号	WOS:000227555900004
公开日期	2010-12-22
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/2449]
专题	海洋研究所_海洋环流与波动重点实验室
作者单位	1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China 3.Chinese Acad Sci, Inst Bot, Beijing 100093, Peoples R China
推荐引用方式 GB/T 7714	Shi, CJ,Hu, ZM,He, YJ,et al. Identification and assessing the cultivars of Laminaria Lamx. (phaeophyceae) with molecular markers[J]. JOURNAL OF INTEGRATIVE PLANT BIOLOGY,2005,47(3):283-291.
APA	Shi, CJ,Hu, ZM,He, YJ,Zou, YP,Zhang, DM,&Duan, DL.(2005).Identification and assessing the cultivars of Laminaria Lamx. (phaeophyceae) with molecular markers.JOURNAL OF INTEGRATIVE PLANT BIOLOGY,47(3),283-291.
MLA	Shi, CJ,et al."Identification and assessing the cultivars of Laminaria Lamx. (phaeophyceae) with molecular markers".JOURNAL OF INTEGRATIVE PLANT BIOLOGY 47.3(2005):283-291.