The responsive expression of heat shock protein 22 gene in zhikong scallop Chlamys farreri against a bacterial challenge

doi:10.1111/j.1365-2109.2009.02328.x

CORC > 海洋研究所 > 中国科学院海洋研究所 > 海洋生态与环境科学重点实验室

	The responsive expression of heat shock protein 22 gene in zhikong scallop Chlamys farreri against a bacterial challenge
	Zhang, Lei 1,2; Wang, Lingling 1; Zhao, Jianmin 1; Qiu, Limei 1; Song, Linsheng 1; Dong, Chaohua 1,2; Li, Fengmei 1; Song, LS, Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, 7 Nanhai Rd, Qingdao 266071, Peoples R China
刊名	AQUACULTURE RESEARCH
	2010-01-18
卷号	41 期号:2 页码:257-266
关键词	Hsp22 Chlamys Farreri Innate Defence System Bacteria Challenge
ISSN号	1355-557X
DOI	10.1111/j.1365-2109.2009.02328.x
文献子类	Article
英文摘要	HSP22 is a member of a small HSP subfamily contributing to the growth, transformation and apoptosis of the cell as well as acting as a molecular chaperone. In the present study, CfHSP22 cDNA was cloned from Chlamys farreri by the rapid amplification of cDNA ends technique. The full-length cDNA of CfHSP22 was of 1279 bp, consisting of a 5'-terminal untranslated region (5'UTR) of 122 bp, a 3'UTR of 581 bp with a canonical polyadenylation signal sequence AATAAA and a poly( A) tail, and an open reading frame of 576 bp encoding a polypeptide with a molecular mass of 22.21 kDa and a predicted isoelectric point of 9.69. There was an alpha-crystallin domain, a hallmark of the sHSP subfamily, in the C-terminus, and the deduced amino acid sequence of CfHSP22 showed high similarity to previously identified HSP22s. CfHSP22 was constitutively expressed in the haemocyte, muscle, kidney, gonad, gill, heart and hepatopancreas, and the expression level in the hepatopancreas was higher than that in the other tissues. CfHSP22 transcription was up-regulated and reached a maximal level at 12 h after the bacterial challenge, and then declined progressively to the original level at 48 h. These results suggested that CfHSP22 perhaps play a critical role in response to the bacterial challenge in haemocytes of scallop C. farreri.; HSP22 is a member of a small HSP subfamily contributing to the growth, transformation and apoptosis of the cell as well as acting as a molecular chaperone. In the present study, CfHSP22 cDNA was cloned from Chlamys farreri by the rapid amplification of cDNA ends technique. The full-length cDNA of CfHSP22 was of 1279 bp, consisting of a 5'-terminal untranslated region (5'UTR) of 122 bp, a 3'UTR of 581 bp with a canonical polyadenylation signal sequence AATAAA and a poly( A) tail, and an open reading frame of 576 bp encoding a polypeptide with a molecular mass of 22.21 kDa and a predicted isoelectric point of 9.69. There was an alpha-crystallin domain, a hallmark of the sHSP subfamily, in the C-terminus, and the deduced amino acid sequence of CfHSP22 showed high similarity to previously identified HSP22s. CfHSP22 was constitutively expressed in the haemocyte, muscle, kidney, gonad, gill, heart and hepatopancreas, and the expression level in the hepatopancreas was higher than that in the other tissues. CfHSP22 transcription was up-regulated and reached a maximal level at 12 h after the bacterial challenge, and then declined progressively to the original level at 48 h. These results suggested that CfHSP22 perhaps play a critical role in response to the bacterial challenge in haemocytes of scallop C. farreri.
学科主题	Fisheries
URL标识	查看原文
语种	英语
WOS记录号	WOS:000273546500011
公开日期	2010-11-18
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/1821]
专题	海洋研究所_海洋生态与环境科学重点实验室
通讯作者	Song, LS, Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, 7 Nanhai Rd, Qingdao 266071, Peoples R China
作者单位	1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Ocean Univ China, Coll Marine Life, Qingdao, Peoples R China
推荐引用方式 GB/T 7714	Zhang, Lei,Wang, Lingling,Zhao, Jianmin,et al. The responsive expression of heat shock protein 22 gene in zhikong scallop Chlamys farreri against a bacterial challenge[J]. AQUACULTURE RESEARCH,2010,41(2):257-266.
APA	Zhang, Lei.,Wang, Lingling.,Zhao, Jianmin.,Qiu, Limei.,Song, Linsheng.,...&Song, LS, Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, 7 Nanhai Rd, Qingdao 266071, Peoples R China.(2010).The responsive expression of heat shock protein 22 gene in zhikong scallop Chlamys farreri against a bacterial challenge.AQUACULTURE RESEARCH,41(2),257-266.
MLA	Zhang, Lei,et al."The responsive expression of heat shock protein 22 gene in zhikong scallop Chlamys farreri against a bacterial challenge".AQUACULTURE RESEARCH 41.2(2010):257-266.