Integrating the Vibrio-resistance phenotype and gene expression data for discovery of markers used for resistance evaluation in the clam Meretrix petechialis

doi:10.1016/j.aquaculture.2017.09.033

CORC > 海洋研究所 > 中国科学院海洋研究所 > 实验海洋生物学重点实验室

	Integrating the Vibrio-resistance phenotype and gene expression data for discovery of markers used for resistance evaluation in the clam Meretrix petechialis
	Jiang, Fengjuan 1,2; Wang, Hongxia 1; Yue, Xin 1; Zhang, Shujing 1,2; Liu, Baozhong1,3
刊名	AQUACULTURE
	2018
卷号	482 页码:130-136
关键词	Meretrix Petechialis Vibrio Parahaemolyticus Resistance Gene Expression Level Molecular Markers
DOI	10.1016/j.aquaculture.2017.09.033
文献子类	Article
英文摘要	In this study, gene expression data were used to select resistance-associated markers that indicate resistance phenotypes in the clam Meretrix petechialis. A total of 1772 clams from 23 full-sibling families were subjected to a Vibrio challenge test, and the results showed significant variation in resistance to vibriosis among different families. Three of the most resistant families and three of the most susceptible families were selected for screening of resistance-associated markers. Then, among 22 genes, 12 demonstrated a significant change in expression, and their expression levels were further analysed by t-test and linear regression analyses, respectively, between resistant and susceptible families. Overall, the results showed that the basal expression levels of Big-Def, BIRC7, NFIL3, CTL9 and Bax were associated with Vibrio-resistant phenotypes and could be considered candidate resistance-associated markers. Further, two M. petechialis strains that differed in Vibrio resistance were used to examine the effectiveness of these five resistance-associated genes. The results further confirmed that the expression of BIRC7 and NFIL3 were associated with Vibrio-resistant phenotypes. This study integrated the Vibrio-resistance phenotype and gene expression data for resistance evaluation and provided potential markers for the genetic breeding of high Vibrio-resistance strains in the clam M. petechialis.
语种	英语
WOS记录号	WOS:000413434400020
内容类型	期刊论文
版本	出版稿
源URL	[http://ir.qdio.ac.cn/handle/337002/143229]
专题	海洋研究所_实验海洋生物学重点实验室
作者单位	1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266000, Peoples R China
推荐引用方式 GB/T 7714	Jiang, Fengjuan,Wang, Hongxia,Yue, Xin,et al. Integrating the Vibrio-resistance phenotype and gene expression data for discovery of markers used for resistance evaluation in the clam Meretrix petechialis[J]. AQUACULTURE,2018,482:130-136.
APA	Jiang, Fengjuan,Wang, Hongxia,Yue, Xin,Zhang, Shujing,&Liu, Baozhong.(2018).Integrating the Vibrio-resistance phenotype and gene expression data for discovery of markers used for resistance evaluation in the clam Meretrix petechialis.AQUACULTURE,482,130-136.
MLA	Jiang, Fengjuan,et al."Integrating the Vibrio-resistance phenotype and gene expression data for discovery of markers used for resistance evaluation in the clam Meretrix petechialis".AQUACULTURE 482(2018):130-136.