MARS: A protein family involved in the formation of vertical skeletal elements

doi:10.1016/j.jsb.2017.04.003

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	MARS: A protein family involved in the formation of vertical skeletal elements
	Abehsera, Shai 1,2; Peles, Shani 1; Tynyakov, Jenny 3; Bentov, Shmuel 2; Aflalo, Eliahu D.1,2; Li, Shihao 4; Li, Fuhua 4; Xiang, Jianhai4 ; Sagi, Amir 1,2
刊名	JOURNAL OF STRUCTURAL BIOLOGY
	2017-05-01
卷号	198 期号:2 页码:92-102
关键词	Crustacean Mandible Chitin Fibers Mars Proteins Structural Proteins Tooth Structure Vertical Elements
DOI	10.1016/j.jsb.2017.04.003
文献子类	Article
英文摘要	Vertical organizations of skeletal elements are found in various vertebrate teeth and invertebrate exoskeletons. The molecular mechanism behind the development of such structural organizations is poorly known, although it is generally held that organic matrix proteins play an essential role. While most crustacean cuticular organizations exhibit horizontal chitinous layering, a typical vertical organization is found towards the surface of the teeth in the mandibles of the crayfish Cherax quadricarinatus. Candidate genes encoding for mandible-forming structural proteins were mined in C quadricarinatus molt-related transcriptomic libraries by using a binary patterning approach. A new protein family, termed the Mandible Alanine Rich Structural (MARS) protein family, with a modular sequence design predicted to form fibers, was found. Investigations of spatial and temporal expression of the different MARS genes suggested specific expression in the mandibular teeth-forming epithelium, particularly during the formation of the chitinous vertical organization. MARS loss-of-function RNAi experiments resulted in the collapse of the organization of the chitin fibers oriented vertically to the surface of the crayfish mandibular incisor tooth. A general search of transcriptomic libraries suggested conservation of MARS proteins across a wide array of crustaceans. Our results provide a first look into the molecular mechanism used to build the complex crustacean mandible and into the specialized vertical structural solution that has evolved in skeletal elements. (C) 2017 Elsevier Inc. All rights reserved.
语种	英语
WOS记录号	WOS:000402026300004
内容类型	期刊论文
版本	出版稿
源URL	[http://ir.qdio.ac.cn/handle/337002/136863]
专题	海洋研究所_实验海洋生物学重点实验室
作者单位	1.Ben Gurion Univ Negev, Dept Life Sci, Beer Sheva, Israel 2.Ben Gurion Univ Negev, Natl Inst Biotechnol Negev, Beer Sheva, Israel 3.Tel Aviv Univ, Dept Zool, Interuniv Inst Marine Sci, Steinitz Marine Biol Lab, Tel Aviv, Israel 4.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao, Peoples R China
推荐引用方式 GB/T 7714	Abehsera, Shai,Peles, Shani,Tynyakov, Jenny,et al. MARS: A protein family involved in the formation of vertical skeletal elements[J]. JOURNAL OF STRUCTURAL BIOLOGY,2017,198(2):92-102.
APA	Abehsera, Shai.,Peles, Shani.,Tynyakov, Jenny.,Bentov, Shmuel.,Aflalo, Eliahu D..,...&Sagi, Amir.(2017).MARS: A protein family involved in the formation of vertical skeletal elements.JOURNAL OF STRUCTURAL BIOLOGY,198(2),92-102.
MLA	Abehsera, Shai,et al."MARS: A protein family involved in the formation of vertical skeletal elements".JOURNAL OF STRUCTURAL BIOLOGY 198.2(2017):92-102.