Photosynthetic responses of thalli and isolated protoplasts of Bryopsis hypnoides (Bryopsidales, Chlorophyta) during dehydration

doi:10.1007/s00343-011-0028-4

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	Photosynthetic responses of thalli and isolated protoplasts of Bryopsis hypnoides (Bryopsidales, Chlorophyta) during dehydration
	Lue Fang 1,2; Wang Guangce 1,3; Jin Haochen 3
刊名	CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY
	2011-03-01
卷号	29 期号:2 页码:334-342
关键词	Bryopsis Hypnoides Chlorophyll Fluorescence Dehydration Protoplasts Pulse Amplified Modulation Fluorescence System
ISSN号	0254-4059
DOI	10.1007/s00343-011-0028-4
文献子类	Article
英文摘要	Bryopsis hypnoides Lamouroux is a unique intertidal siphonous green alga whose extruded protoplasm can aggregate spontaneously in seawater to form numerous new cells that can develop into mature algal thalli. In this study, the photosynthetic responses during dehydration of both the thalli and protoplasts isolated from B. hypnoides were measured using a Dual-PAM (pulse amplitude modulation)-100 fluorometer. The results show that the photosynthetic rates of B. hypnoides thalli were maintained for an initial period, beyond which continued desiccation resulted in reduced rates of PSI and PSII. However, the photosynthetic performances of the isolated protoplasts dehydrated in air (CO(2) concentration 600-700 mg/L) showed a slight increase of Y(II) at 20% water loss, but the rates decreased thereafter with declining water content. When protoplasts were dehydrated in CO(2) deficient conditions (CO(2) concentration 40 80 mg/L), the values of Y(II) declined steadily with increased dehydration without an initial rise. These results indicated that the thalli and isolated protoplasts of this alga can utilize CO(2) in ambient air effectively, and the photosynthetic performances of the isolated protoplasts were significantly different from that of the thalli during dehydration. Thus the protoplasts may be an excellent system for the study of stress tolerance.; Bryopsis hypnoides Lamouroux is a unique intertidal siphonous green alga whose extruded protoplasm can aggregate spontaneously in seawater to form numerous new cells that can develop into mature algal thalli. In this study, the photosynthetic responses during dehydration of both the thalli and protoplasts isolated from B. hypnoides were measured using a Dual-PAM (pulse amplitude modulation)-100 fluorometer. The results show that the photosynthetic rates of B. hypnoides thalli were maintained for an initial period, beyond which continued desiccation resulted in reduced rates of PSI and PSII. However, the photosynthetic performances of the isolated protoplasts dehydrated in air (CO(2) concentration 600-700 mg/L) showed a slight increase of Y(II) at 20% water loss, but the rates decreased thereafter with declining water content. When protoplasts were dehydrated in CO(2) deficient conditions (CO(2) concentration 40 80 mg/L), the values of Y(II) declined steadily with increased dehydration without an initial rise. These results indicated that the thalli and isolated protoplasts of this alga can utilize CO(2) in ambient air effectively, and the photosynthetic performances of the isolated protoplasts were significantly different from that of the thalli during dehydration. Thus the protoplasts may be an excellent system for the study of stress tolerance.
学科主题	Marine & Freshwater Biology ; Oceanography
URL标识	查看原文
语种	英语
WOS记录号	WOS:000287924000013
公开日期	2012-07-03
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/11916]
专题	海洋研究所_实验海洋生物学重点实验室
作者单位	1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China 2.Grad Univ, Chinese Acad Sci, Beijing 100049, Peoples R China 3.Tianjin Univ Sci & Technol, Coll Marine Sci & Engn, Tianjin 300457, Peoples R China
推荐引用方式 GB/T 7714	Lue Fang,Wang Guangce,Jin Haochen. Photosynthetic responses of thalli and isolated protoplasts of Bryopsis hypnoides (Bryopsidales, Chlorophyta) during dehydration[J]. CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY,2011,29(2):334-342.
APA	Lue Fang,Wang Guangce,&Jin Haochen.(2011).Photosynthetic responses of thalli and isolated protoplasts of Bryopsis hypnoides (Bryopsidales, Chlorophyta) during dehydration.CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY,29(2),334-342.
MLA	Lue Fang,et al."Photosynthetic responses of thalli and isolated protoplasts of Bryopsis hypnoides (Bryopsidales, Chlorophyta) during dehydration".CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY 29.2(2011):334-342.