A primitive Toll-like receptor signaling pathway in mollusk Zhikong scallop Chlamys farreri

doi:10.1016/j.dci.2010.12.005

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	A primitive Toll-like receptor signaling pathway in mollusk Zhikong scallop Chlamys farreri
	Wang, Mengqiang1,2 ; Yang, Jialong 1,2; Zhou, Zhi 1,2; Qiu, Limei 1; Wang, Lingling ; Zhang, Huan 1; Gao, Yang1,2 ; Wang, Xingqiang 1; Zhang, Li 1; Zhao, Jianmin 1
刊名	DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
	2011-04-01
卷号	35 期号:4 页码:511-520
关键词	Chlamys Farreri Innate Immunity Tlr Signaling Pathway
ISSN号	0145-305X
DOI	10.1016/j.dci.2010.12.005
文献子类	Article
英文摘要	As a member of pattern-recognition receptors (PRRs), the Toll-like receptor (TLR) and its signaling pathway play pivotal roles in recognizing various pathogen-associated molecular patterns (PAMPs), and buildup the front-line against invading pathogens. In the present study, the sequence features and mRNA expression profiles of five key genes involved in TLR signal pathway were characterized, and their functions in the immune responses were also investigated in order to validate the TLR signaling pathway and its potential roles in the immune defense of Zhikong scallop Chlamys farreri. These five genes, including MLR, CfMyD88, CfTRAF6, Cfl kappa B and CfNF kappa B, exhibited significant similarity with their homologues from other model organisms, and contained the typical motifs. A strong interaction between the TIR domain from CfTLR and CfMyD88 protein was revealed via ELISA assays. The mRNA transcripts of these five genes were all up-regulated after LPS stimulation, indicating that they were involved in the immune response against LPS. When CfTLR expression was inhibited by RNAi technology, the mRNA expression level of CfMyD88, CfTRAF6, Cfl kappa B, CfNF kappa B and G-type lysozyme were all decreased, while those of superoxide dismutase and catalase were increased. After Listonella anguillara challenge, the apoptosis level of those MLR-suppressed scallops was significantly lower than that in control groups (p < 0.05) at the beginning of bacteria challenge, while the cumulative mortality was significantly higher than that of control groups (p < 0.05). These results collectively favored that a rather canonical MyD88-dependent TLR pathway existed in scallop and this pathway was involved in immune signaling to active the diverse downstream reaction including anti-oxidant, anti-bacteria and apoptosis. (C) 2010 Elsevier Ltd. All rights reserved.; As a member of pattern-recognition receptors (PRRs), the Toll-like receptor (TLR) and its signaling pathway play pivotal roles in recognizing various pathogen-associated molecular patterns (PAMPs), and buildup the front-line against invading pathogens. In the present study, the sequence features and mRNA expression profiles of five key genes involved in TLR signal pathway were characterized, and their functions in the immune responses were also investigated in order to validate the TLR signaling pathway and its potential roles in the immune defense of Zhikong scallop Chlamys farreri. These five genes, including MLR, CfMyD88, CfTRAF6, Cfl kappa B and CfNF kappa B, exhibited significant similarity with their homologues from other model organisms, and contained the typical motifs. A strong interaction between the TIR domain from CfTLR and CfMyD88 protein was revealed via ELISA assays. The mRNA transcripts of these five genes were all up-regulated after LPS stimulation, indicating that they were involved in the immune response against LPS. When CfTLR expression was inhibited by RNAi technology, the mRNA expression level of CfMyD88, CfTRAF6, Cfl kappa B, CfNF kappa B and G-type lysozyme were all decreased, while those of superoxide dismutase and catalase were increased. After Listonella anguillara challenge, the apoptosis level of those MLR-suppressed scallops was significantly lower than that in control groups (p < 0.05) at the beginning of bacteria challenge, while the cumulative mortality was significantly higher than that of control groups (p < 0.05). These results collectively favored that a rather canonical MyD88-dependent TLR pathway existed in scallop and this pathway was involved in immune signaling to active the diverse downstream reaction including anti-oxidant, anti-bacteria and apoptosis. (C) 2010 Elsevier Ltd. All rights reserved.
学科主题	Immunology ; Zoology
URL标识	查看原文
语种	英语
WOS记录号	WOS:000288819800013
公开日期	2012-07-03
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/11764]
专题	海洋研究所_实验海洋生物学重点实验室海洋研究所_海洋腐蚀与防护研究发展中心
作者单位	1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Shandong, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Wang, Mengqiang,Yang, Jialong,Zhou, Zhi,et al. A primitive Toll-like receptor signaling pathway in mollusk Zhikong scallop Chlamys farreri[J]. DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,2011,35(4):511-520.
APA	Wang, Mengqiang.,Yang, Jialong.,Zhou, Zhi.,Qiu, Limei.,Wang, Lingling.,...&Song, Linsheng.(2011).A primitive Toll-like receptor signaling pathway in mollusk Zhikong scallop Chlamys farreri.DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,35(4),511-520.
MLA	Wang, Mengqiang,et al."A primitive Toll-like receptor signaling pathway in mollusk Zhikong scallop Chlamys farreri".DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY 35.4(2011):511-520.