Species identification in salted products of red snappers by semi-nested PCR-RFLP based on the mitochondrial 12S rRNA gene sequence

doi:10.1016/j.foodcont.2005.01.011

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	Species identification in salted products of red snappers by semi-nested PCR-RFLP based on the mitochondrial 12S rRNA gene sequence
	Zhang, JB ; Huang, H ; Cai, ZP ; Huang, LM
刊名	FOOD CONTROL
	2006-07-01
卷号	17 期号:7 页码:557-563
关键词	Identification Red Snappers Semi-nested Pcr 12s Rrna Gene
ISSN号	0956-7135
DOI	10.1016/j.foodcont.2005.01.011
文献子类	Article
英文摘要	A molecular approach was developed to distinguish species of red snappers among commercial salted fish products. The specific fragments of the mitochondrial 12S rRNA gene, which were about 450bp, were obtained using the semi-nested polymerase chain reaction (semi-nested PCR). Subsequently, PCR arnplicons were sequenced, aiming to select restriction endonucleases that generated species-specific restriction fragment length polymorphism (RFLP) profiles. Discrimination of red snappers Lutjanus sanguineus, Lutjanus erythopterus from Lutjanus argentimaculatus, Lutjanus malabarius and other morphologically similar fishes such as Lethrinus leutjanus and Pinjalo pinjalo was feasible by one restriction digestion reaction with three endonucleases Hae III, Sca I and SnaB I, however, for discrimination of L. sanguineus and L. erythopterus, another restriction digestion reaction with single restriction endonuclease Mae II was needed. The semi-nested PCR-RFLP was demonstrated to be reliable in species identification of salted fish products in this study. (c) 2005 Elsevier Ltd. All rights reserved.; A molecular approach was developed to distinguish species of red snappers among commercial salted fish products. The specific fragments of the mitochondrial 12S rRNA gene, which were about 450bp, were obtained using the semi-nested polymerase chain reaction (semi-nested PCR). Subsequently, PCR arnplicons were sequenced, aiming to select restriction endonucleases that generated species-specific restriction fragment length polymorphism (RFLP) profiles. Discrimination of red snappers Lutjanus sanguineus, Lutjanus erythopterus from Lutjanus argentimaculatus, Lutjanus malabarius and other morphologically similar fishes such as Lethrinus leutjanus and Pinjalo pinjalo was feasible by one restriction digestion reaction with three endonucleases Hae III, Sca I and SnaB I, however, for discrimination of L. sanguineus and L. erythopterus, another restriction digestion reaction with single restriction endonuclease Mae II was needed. The semi-nested PCR-RFLP was demonstrated to be reliable in species identification of salted fish products in this study. (c) 2005 Elsevier Ltd. All rights reserved.
学科主题	Food Science & Technology
URL标识	查看原文
语种	英语
WOS记录号	WOS:000235266600009
公开日期	2010-12-24
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/5985]
专题	海洋研究所_实验海洋生物学重点实验室
作者单位	1.Chinese Acad Sci, S China Sea Inst Oceanog, LED, Guangzhou 510301, Peoples R China 2.Chinese Acad Sci, Inst Oceanog, Qingdao 266071, Peoples R China
推荐引用方式 GB/T 7714	Zhang, JB,Huang, H,Cai, ZP,et al. Species identification in salted products of red snappers by semi-nested PCR-RFLP based on the mitochondrial 12S rRNA gene sequence[J]. FOOD CONTROL,2006,17(7):557-563.
APA	Zhang, JB,Huang, H,Cai, ZP,&Huang, LM.(2006).Species identification in salted products of red snappers by semi-nested PCR-RFLP based on the mitochondrial 12S rRNA gene sequence.FOOD CONTROL,17(7),557-563.
MLA	Zhang, JB,et al."Species identification in salted products of red snappers by semi-nested PCR-RFLP based on the mitochondrial 12S rRNA gene sequence".FOOD CONTROL 17.7(2006):557-563.