The cDNA cloning and mRNA expression of a potential selenium-binding protein gene in the scallop Chlamys farreri

doi:10.1016/j.dci.2005.04.001

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	The cDNA cloning and mRNA expression of a potential selenium-binding protein gene in the scallop Chlamys farreri
	Song, LS ; Zou, HB ; Chang, YQ ; Xu, W ; Wu, LT
刊名	DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
	2006
卷号	30 期号:3 页码:265-273
关键词	Chlamys Farreri Expressed Sequence Tag Selenium Binding Protein Cdna Cloning Mrna Expression Oxidative Stress Immunity Response
ISSN号	0145-305X
DOI	10.1016/j.dci.2005.04.001
文献子类	Article
英文摘要	Selenium binding proteins (SeBP) represent a family of proteins that are believed to be involved in controlling the oxidation/reduction in many physiological processes. The cDNA of Zhikong Scallop Chlamys farreri selenium binding protein (zSeBP) was cloned by expressed sequence tag (EST) and RACE techniques. The high similarity of zSeBP deduced amino acid sequence with the SeBP in other organisms, such as bird, fish, frog, mosquito, fruit fly, mammalian, and even nematode and microorganism indicated that zSeBP should be a member of SeBP family. The temporal expression of zSeBP in the hemocytes was measured by semi-quantitative RT-PCR after scallops were stimulated by either oxidative stress or microbial challenge. The expression of zSeBP was up-regulated progressively after stimulation, and then dropped gradually to the original level. Meanwhile, malondialdehyde (MDA) measured by the colorimetric method in the microbial challenged scallops increased immediately after scallops was challenged by microbes, and was significantly higher than that in the control scallops. Results indicated that the microbial infection could incense the disorder of oxidation/reduction and may result in high MDA production. The negative correlation between the expression level of zSeBP and the MDA content suggested that zSeBP could play an important role in mediating the anti-oxidation mechanisms and immune response in marine invertebrates. (c) 2005 Published by Elsevier Ltd.; Selenium binding proteins (SeBP) represent a family of proteins that are believed to be involved in controlling the oxidation/reduction in many physiological processes. The cDNA of Zhikong Scallop Chlamys farreri selenium binding protein (zSeBP) was cloned by expressed sequence tag (EST) and RACE techniques. The high similarity of zSeBP deduced amino acid sequence with the SeBP in other organisms, such as bird, fish, frog, mosquito, fruit fly, mammalian, and even nematode and microorganism indicated that zSeBP should be a member of SeBP family. The temporal expression of zSeBP in the hemocytes was measured by semi-quantitative RT-PCR after scallops were stimulated by either oxidative stress or microbial challenge. The expression of zSeBP was up-regulated progressively after stimulation, and then dropped gradually to the original level. Meanwhile, malondialdehyde (MDA) measured by the colorimetric method in the microbial challenged scallops increased immediately after scallops was challenged by microbes, and was significantly higher than that in the control scallops. Results indicated that the microbial infection could incense the disorder of oxidation/reduction and may result in high MDA production. The negative correlation between the expression level of zSeBP and the MDA content suggested that zSeBP could play an important role in mediating the anti-oxidation mechanisms and immune response in marine invertebrates. (c) 2005 Published by Elsevier Ltd.
学科主题	Immunology ; Zoology
URL标识	查看原文
语种	英语
WOS记录号	WOS:000234732500003
公开日期	2010-12-24
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/5921]
专题	海洋研究所_实验海洋生物学重点实验室
作者单位	1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China 2.Ocean Univ China, Qingdao 266071, Peoples R China 3.Dalian Fisheries Coll, Dalian 116023, Peoples R China
推荐引用方式 GB/T 7714	Song, LS,Zou, HB,Chang, YQ,et al. The cDNA cloning and mRNA expression of a potential selenium-binding protein gene in the scallop Chlamys farreri[J]. DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,2006,30(3):265-273.
APA	Song, LS,Zou, HB,Chang, YQ,Xu, W,&Wu, LT.(2006).The cDNA cloning and mRNA expression of a potential selenium-binding protein gene in the scallop Chlamys farreri.DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,30(3),265-273.
MLA	Song, LS,et al."The cDNA cloning and mRNA expression of a potential selenium-binding protein gene in the scallop Chlamys farreri".DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY 30.3(2006):265-273.