Comparative studies on the efficiency of energy transfer of two R-phycoerythrin-C-phycocyanin conjugates constructed from sodium periodate and glutaraldehyde respectively

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	Comparative studies on the efficiency of energy transfer of two R-phycoerythrin-C-phycocyanin conjugates constructed from sodium periodate and glutaraldehyde respectively
	Wang, GC; Ma, SY; Zeng, CK
刊名	PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS
	2004-03-01
卷号	31 期号:3 页码:273-277
关键词	R-phycoerythrin C-phycocyanin Crosslink By Oxidation With Sodium Periodate Crosslink With Glutaraldehyde Conjugate Spectra Analysis Energy Transfer Efficiency
ISSN号	1000-3282
文献子类	Article
英文摘要	The C-phycocyanin and the R-phycoerythrin were purified from the blue-green alga Spirulina platensis and red alga Polysiphonia urceolata respectively. Both sodium periodate and glutaraldehyde are effective coupling agents being capable of constructing the R-phycoerythrin-C-phycocyanin conjugate, which was also called phycobiliproteins energy transfer model. The two artificial conjugates constructed with different methods were purified by Sephadex G-200 chromatography respectively. Spectra analysis indicated that energy transfer occurred in the two conjugates. The conjugate with sodium periodate had the higher efficiency of energy transfer than that with glutaraldehyde conjugate.; The C-phycocyanin and the R-phycoerythrin were purified from the blue-green alga Spirulina platensis and red alga Polysiphonia urceolata respectively. Both sodium periodate and glutaraldehyde are effective coupling agents being capable of constructing the R-phycoerythrin-C-phycocyanin conjugate, which was also called phycobiliproteins energy transfer model. The two artificial conjugates constructed with different methods were purified by Sephadex G-200 chromatography respectively. Spectra analysis indicated that energy transfer occurred in the two conjugates. The conjugate with sodium periodate had the higher efficiency of energy transfer than that with glutaraldehyde conjugate.
学科主题	Biochemistry & Molecular Biology ; Biophysics
语种	英语
WOS记录号	WOS:000224108600015
公开日期	2010-12-22
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/3227]
专题	海洋研究所_实验海洋生物学重点实验室
作者单位	Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China
推荐引用方式 GB/T 7714	Wang, GC,Ma, SY,Zeng, CK. Comparative studies on the efficiency of energy transfer of two R-phycoerythrin-C-phycocyanin conjugates constructed from sodium periodate and glutaraldehyde respectively[J]. PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS,2004,31(3):273-277.
APA	Wang, GC,Ma, SY,&Zeng, CK.(2004).Comparative studies on the efficiency of energy transfer of two R-phycoerythrin-C-phycocyanin conjugates constructed from sodium periodate and glutaraldehyde respectively.PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS,31(3),273-277.
MLA	Wang, GC,et al."Comparative studies on the efficiency of energy transfer of two R-phycoerythrin-C-phycocyanin conjugates constructed from sodium periodate and glutaraldehyde respectively".PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS 31.3(2004):273-277.