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	A new method for screening alpha-glucosidase inhibitors and application to marine microorganisms
	Chen, HM; Yan, XJ; Lin, W; Zheng, L; Zhang, WW
刊名	PHARMACEUTICAL BIOLOGY
	2004-09-01
卷号	42期号:6页码:416-421
关键词	Enzyme Assay Alpha-glucosidase Inhibitor Marine Microorganism
ISSN号	1388-0209
DOI	10.1080/13880200490885987
文献子类	Article
英文摘要	A new enzyme assay method for screening alpha-glucosidase inhibitors with rapidity and simplicity was developed. The enzyme-substituted alpha-glucosidases for this assay was glucoamylase. Samples were spotted or developed on the silica gel plate. The agar solution containing substrate was poured on the plate, and paper impregnated with enzyme was layered on the agar. After incubation, an inhibitory circle would appear around the inhibitor. By using this method, more than 200 strains of marine microorganisms were screened. Among them, three active strains were found to secrete inhibitors in the culture medium.; A new enzyme assay method for screening alpha-glucosidase inhibitors with rapidity and simplicity was developed. The enzyme-substituted alpha-glucosidases for this assay was glucoamylase. Samples were spotted or developed on the silica gel plate. The agar solution containing substrate was poured on the plate, and paper impregnated with enzyme was layered on the agar. After incubation, an inhibitory circle would appear around the inhibitor. By using this method, more than 200 strains of marine microorganisms were screened. Among them, three active strains were found to secrete inhibitors in the culture medium.
学科主题	Plant Sciences ; Medical Laboratory Technology ; Pharmacology & Pharmacy
URL标识	查看原文
语种	英语
WOS记录号	WOS:000226444900006
公开日期	2010-12-22
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/3179]
专题	海洋研究所_实验海洋生物学重点实验室
作者单位	1.Ningbo Univ, Key Lab Marine Biotechnol, Ningbo 315211, Peoples R China 2.Chinese Acad Sci, Inst Oceanol, Qingdao, Peoples R China
推荐引用方式 GB/T 7714	Chen, HM,Yan, XJ,Lin, W,et al. A new method for screening alpha-glucosidase inhibitors and application to marine microorganisms[J]. PHARMACEUTICAL BIOLOGY,2004,42(6):416-421.
APA	Chen, HM,Yan, XJ,Lin, W,Zheng, L,&Zhang, WW.(2004).A new method for screening alpha-glucosidase inhibitors and application to marine microorganisms.PHARMACEUTICAL BIOLOGY,42(6),416-421.
MLA	Chen, HM,et al."A new method for screening alpha-glucosidase inhibitors and application to marine microorganisms".PHARMACEUTICAL BIOLOGY 42.6(2004):416-421.

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