One-step chromatography method for efficient separation and purification of R-phycoerythrin from Polysiphonia urceolata | |
Liu, LN; Chen, XL; Zhang, XY![]() | |
刊名 | JOURNAL OF BIOTECHNOLOGY
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2005-03-02 | |
卷号 | 116期号:1页码:91-100 |
关键词 | Polysiphonia Urceolata R-phycoerythrin Separation Purification Ion-exchange Chromatography |
ISSN号 | 0168-1656 |
DOI | 10.1016/j.jbiotec.2004.09.017 |
文献子类 | Article |
英文摘要 | Phycoerythrins have been widely used in food, cosmetics., immunodiagnostics and analytical reagents. An efficient one-step chromatography method for purification of R-phycoerythrins from Polysiphonia urceolata was described in this paper. Pure R-phycoerythrin was obtained with an absorbance ratio A(565)/A(280) of 5.6 and a high recovery yield of 67-33%, using a DEAE-Sepharose Fast Flow chromatography with a gradient elution of pH, alternative to common gradient elution of ionic strength. The absorption spectrum of R-phycoerythrin was characterized with three absorbance maxima at 565, 539 and 498 mum, respectively and the fluorescence emission spectrum at room temperature was measured to be 580nm. The results of native-PAGE. and SDS-PAGE showed no contamination by other proteins in the phycoerythrin solution. which suggests an efficient method for the separation and purification of R-phycoerythrins from Polysiphonia urceolata. (C) 2004 Elsevier B.V. All rights reserved.; Phycoerythrins have been widely used in food, cosmetics., immunodiagnostics and analytical reagents. An efficient one-step chromatography method for purification of R-phycoerythrins from Polysiphonia urceolata was described in this paper. Pure R-phycoerythrin was obtained with an absorbance ratio A(565)/A(280) of 5.6 and a high recovery yield of 67-33%, using a DEAE-Sepharose Fast Flow chromatography with a gradient elution of pH, alternative to common gradient elution of ionic strength. The absorption spectrum of R-phycoerythrin was characterized with three absorbance maxima at 565, 539 and 498 mum, respectively and the fluorescence emission spectrum at room temperature was measured to be 580nm. The results of native-PAGE. and SDS-PAGE showed no contamination by other proteins in the phycoerythrin solution. which suggests an efficient method for the separation and purification of R-phycoerythrins from Polysiphonia urceolata. (C) 2004 Elsevier B.V. All rights reserved. |
学科主题 | Biotechnology & Applied Microbiology |
URL标识 | 查看原文 |
语种 | 英语 |
WOS记录号 | WOS:000226711200008 |
公开日期 | 2010-12-22 |
内容类型 | 期刊论文 |
源URL | [http://ir.qdio.ac.cn/handle/337002/3111] ![]() |
专题 | 海洋研究所_实验海洋生物学重点实验室 |
作者单位 | 1.Shandong Univ, State Key Lab Microbial Technol, Jinan 250100, Peoples R China 2.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China |
推荐引用方式 GB/T 7714 | Liu, LN,Chen, XL,Zhang, XY,et al. One-step chromatography method for efficient separation and purification of R-phycoerythrin from Polysiphonia urceolata[J]. JOURNAL OF BIOTECHNOLOGY,2005,116(1):91-100. |
APA | Liu, LN,Chen, XL,Zhang, XY,Zhang, YZ,&Zhou, BC.(2005).One-step chromatography method for efficient separation and purification of R-phycoerythrin from Polysiphonia urceolata.JOURNAL OF BIOTECHNOLOGY,116(1),91-100. |
MLA | Liu, LN,et al."One-step chromatography method for efficient separation and purification of R-phycoerythrin from Polysiphonia urceolata".JOURNAL OF BIOTECHNOLOGY 116.1(2005):91-100. |
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