Pilot-scale fermentation and purification of the recombinant allophycocyanin over-expressed in Escherichia coli

doi:10.1007/s10529-005-5794-7

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	Pilot-scale fermentation and purification of the recombinant allophycocyanin over-expressed in Escherichia coli
	Ge, BS ; Tang, ZH ; Lin, LP ; Ren, YH ; Yang, Y; Qin, S
刊名	BIOTECHNOLOGY LETTERS
	2005-06-01
卷号	27 期号:11 页码:783-787
关键词	Allophycocyanin Escherichia Coli Protein Purification Recombinant Allophycocyanin Tumor Inhibition
ISSN号	0141-5492
DOI	10.1007/s10529-005-5794-7
文献子类	Article
英文摘要	A recombinant allophycocyanin (rAPC), used for treatment of tumors, has been expressed in E. coli which was grown in glucose fed-batch culture in a 30 l fermentor. Recombinant allophycocyanin was purified from soluble E. coli cell lysate using hydrophobic interaction chromatography followed by chromatography using amylose affinity column. The purity of product was greater than 98% and yielded an average of 5.5 g kg(-1) dry cells. Recombinant allophycocyanin significantly inhibited H-22 hepatoma (p (0.01) in mice with inhibition rates ranging from 36% to 62% with doses from 6.25 to 50 mg kg(-1) d(-1).; A recombinant allophycocyanin (rAPC), used for treatment of tumors, has been expressed in E. coli which was grown in glucose fed-batch culture in a 30 l fermentor. Recombinant allophycocyanin was purified from soluble E. coli cell lysate using hydrophobic interaction chromatography followed by chromatography using amylose affinity column. The purity of product was greater than 98% and yielded an average of 5.5 g kg(-1) dry cells. Recombinant allophycocyanin significantly inhibited H-22 hepatoma (p (0.01) in mice with inhibition rates ranging from 36% to 62% with doses from 6.25 to 50 mg kg(-1) d(-1).
学科主题	Biotechnology & Applied Microbiology
URL标识	查看原文
语种	英语
WOS记录号	WOS:000231065100008
公开日期	2010-12-22
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/3099]
专题	海洋研究所_实验海洋生物学重点实验室
作者单位	1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China 3.Chinese Acad Sci, Inst Mat Med, Shanghai 201203, Peoples R China
推荐引用方式 GB/T 7714	Ge, BS,Tang, ZH,Lin, LP,et al. Pilot-scale fermentation and purification of the recombinant allophycocyanin over-expressed in Escherichia coli[J]. BIOTECHNOLOGY LETTERS,2005,27(11):783-787.
APA	Ge, BS,Tang, ZH,Lin, LP,Ren, YH,Yang, Y,&Qin, S.(2005).Pilot-scale fermentation and purification of the recombinant allophycocyanin over-expressed in Escherichia coli.BIOTECHNOLOGY LETTERS,27(11),783-787.
MLA	Ge, BS,et al."Pilot-scale fermentation and purification of the recombinant allophycocyanin over-expressed in Escherichia coli".BIOTECHNOLOGY LETTERS 27.11(2005):783-787.