Molecular analysis of the copper-responsive CopRSCD of a pathogenic Pseudomonas fluorescens strain

doi:10.1007/s12275-008-0278-9

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	Molecular analysis of the copper-responsive CopRSCD of a pathogenic Pseudomonas fluorescens strain
	Hu, Yong-hua 1,2; Wang, Hua-lei 3; Zhang, Min 1,2; Sun, Li 1
刊名	JOURNAL OF MICROBIOLOGY
	2009-06-01
卷号	47 期号:3 页码:277-286
关键词	Copabcd Copper Resistance Copr Pseudomonas Fluorescens Virulence
ISSN号	1225-8873
DOI	10.1007/s12275-008-0278-9
文献子类	Article
英文摘要	CopRS/CopABCD is one of the known systems that control copper homeostasis in bacteria. Although CopRS/CopABCD homologues are found to exist in Pseudomonas fluorescens, the potential role of this system in P. fluorescens has not been investigated. In this study a genetic cluster, consisting of copR, S, C, and D but lacking copAB, was identified in a pathogenic P. fluorescens strain (TSS) isolated from diseased fish. The copRSCD cluster was demonstrated to be required for full copper resistance and regulated at the transcription level by Cu. Expression of copCD is regulated directly by the two-component response regulator CopR, which also regulates its own expression. Interruption of the regulated expression of copR affected bacterial growth, biofilm formation, and tissue dissemination and survival. A mutant CopR, which lacks the N-terminal signal receiver domain and is constitutively active, was found to have an attenuating effect on bacterial virulence when expressed in TSS. To our knowledge, this is the first report that suggests a link between CopR and bacterial pathogenicity in P. fluorescens.; CopRS/CopABCD is one of the known systems that control copper homeostasis in bacteria. Although CopRS/CopABCD homologues are found to exist in Pseudomonas fluorescens, the potential role of this system in P. fluorescens has not been investigated. In this study a genetic cluster, consisting of copR, S, C, and D but lacking copAB, was identified in a pathogenic P. fluorescens strain (TSS) isolated from diseased fish. The copRSCD cluster was demonstrated to be required for full copper resistance and regulated at the transcription level by Cu. Expression of copCD is regulated directly by the two-component response regulator CopR, which also regulates its own expression. Interruption of the regulated expression of copR affected bacterial growth, biofilm formation, and tissue dissemination and survival. A mutant CopR, which lacks the N-terminal signal receiver domain and is constitutively active, was found to have an attenuating effect on bacterial virulence when expressed in TSS. To our knowledge, this is the first report that suggests a link between CopR and bacterial pathogenicity in P. fluorescens.
学科主题	Microbiology
URL标识	查看原文
语种	英语
WOS记录号	WOS:000267396900008
公开日期	2010-12-22
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/2921]
专题	海洋研究所_实验海洋生物学重点实验室
作者单位	1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China 3.Hang Zhou EnzySource Biotechnol Co Ltd, Hangzhou 310030, Zhejiang, Peoples R China
推荐引用方式 GB/T 7714	Hu, Yong-hua,Wang, Hua-lei,Zhang, Min,et al. Molecular analysis of the copper-responsive CopRSCD of a pathogenic Pseudomonas fluorescens strain[J]. JOURNAL OF MICROBIOLOGY,2009,47(3):277-286.
APA	Hu, Yong-hua,Wang, Hua-lei,Zhang, Min,&Sun, Li.(2009).Molecular analysis of the copper-responsive CopRSCD of a pathogenic Pseudomonas fluorescens strain.JOURNAL OF MICROBIOLOGY,47(3),277-286.
MLA	Hu, Yong-hua,et al."Molecular analysis of the copper-responsive CopRSCD of a pathogenic Pseudomonas fluorescens strain".JOURNAL OF MICROBIOLOGY 47.3(2009):277-286.