Affinity chromatography with immobilized DNA stationary phase for biological fingerprinting analysis of traditional Chinese medicines

	Affinity chromatography with immobilized DNA stationary phase for biological fingerprinting analysis of traditional Chinese medicines
	Su, Xingye; Hu, Lianghai; Kong, Liang; Lei, Xiaoyuan; Zou, Hanfa
刊名	journal of chromatography a
	2007-06-22
卷号	1154 期号:1-2 页码:132-137
关键词	affinity chromatography biological fingerprinting analysis immobilized DNA stationary phase traditional Chinese medicines
ISSN号	0021-9673
通讯作者	邹汉法
产权排序	1;1
英文摘要	a stationary phase for high performance affinity chromatography with immobilization of dna onto silica gel was prepared and characterized. the effect of the ionic strength, concentration of mg2+, edta and ch3 cn in the mobile phase on the retention of alkaloids were investigated. with this stationary phase, biological fingerprinting analysis of traditional chinese medicines (tcms) coptis chinensis franch and rheum palmatum l. was performed with both one-dimensional (1-d) and two-dimensional (2-d) chromatography. the 1-d chromatography was performed with isocratic and gradient elution and 2-d chromatography was developed with immobilized dna column combined with silica monolithic ods column. it was found that 7 compounds in coptis chinensis franch including berberine, palmatine and jatrorrhizine, 14 compounds in rheum palmatum l. including aloe-emodin, rhein, emodin, chrysophannol-8-o-glucophranoside and physionl-8-o-glucophranoside were active in binding to the immobilized dna. (c) 2007 elsevier b.v all rights reserved.
WOS标题词	science & technology ; life sciences & biomedicine ; physical sciences
类目[WOS]	biochemical research methods ; chemistry, analytical
研究领域[WOS]	biochemistry & molecular biology ; chemistry
关键词[WOS]	l-rna aptamer ; bioactive compounds ; angelica-sinensis ; in-vitro ; binding ; target ; purification ; spectrometry ; selectivity ; separation
收录类别	SCI
原文出处	true
语种	英语
WOS记录号	WOS:000247419300016
公开日期	2010-11-30
内容类型	期刊论文
源URL	[http://159.226.238.44/handle/321008/98615]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
作者单位	Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Dalian 116023, Peoples R China
推荐引用方式 GB/T 7714	Su, Xingye,Hu, Lianghai,Kong, Liang,et al. Affinity chromatography with immobilized DNA stationary phase for biological fingerprinting analysis of traditional Chinese medicines[J]. journal of chromatography a,2007,1154(1-2):132-137.
APA	Su, Xingye,Hu, Lianghai,Kong, Liang,Lei, Xiaoyuan,&Zou, Hanfa.(2007).Affinity chromatography with immobilized DNA stationary phase for biological fingerprinting analysis of traditional Chinese medicines.journal of chromatography a,1154(1-2),132-137.
MLA	Su, Xingye,et al."Affinity chromatography with immobilized DNA stationary phase for biological fingerprinting analysis of traditional Chinese medicines".journal of chromatography a 1154.1-2(2007):132-137.