Interactions of dextran sulfates with granulocyte colony-stimulating factor and their effects on leukemia cells

	Interactions of dextran sulfates with granulocyte colony-stimulating factor and their effects on leukemia cells
	Liang, Aiye ; Zhou, Xiaomian ; Wang, Qiuyan ; Liu, Xiu ; Qin, Jianhua ; Du, Yuguang ; Wang, Keyi ; Lin, Bingcheng
刊名	electrophoresis
	2006-08-01
卷号	27 期号:16 页码:3195-3201
关键词	capillary zone electrophoresis dextran sulfate granulocyte colony-stimulating factor interaction leukemia cells
通讯作者	林炳承
产权排序	1;1
英文摘要	the interactions between granulocyte colony-stimulating factor (g-csf) and dextran sulfate (ds) with different chain lengths and sulfate contents were studied by capillary zone electrophoresis. it was found that ds with a molecular mass of 500 kda (ds500) could bind to g-csf and the binding constant and binding sites were determined using scatchard plot to be 1.17 x 10(6) m(-1) and 8, respectively. ds with a molecular mass of 40 kda also had the affinity to g-csf and the binding constant and binding sties were 1.01 x 10(6) m-1 and 8, respectively. however, ds with a molecular mass of 8 kda and the non-sulfated saccharide, dextran, had no affinity to g-csf the results indicate that the interactions between g-csif and ds are dependent on the chain lengths and sulfate contents of the saccharides. in addition, the effects of g-csf-binding ds on a g-csf-dependent leukemia cell line were investigated using biological methods. results show that ds500 plus g-csf has potential therapeutic effect on cancers because their combination could inhibit the growth and induce the differentiation of the leukemia cells.
WOS标题词	science & technology ; life sciences & biomedicine ; physical sciences
类目[WOS]	biochemical research methods ; chemistry, analytical
研究领域[WOS]	biochemistry & molecular biology ; chemistry
关键词[WOS]	fibroblast growth-factor ; heparan-sulfate ; capillary-electrophoresis ; binding ; proteoglycans ; differentiation ; receptor ; complex ; protein ; nfs-60
收录类别	SCI
原文出处	true
语种	英语
WOS记录号	WOS:000240333600005
公开日期	2010-11-30
内容类型	期刊论文
源URL	[http://159.226.238.44/handle/321008/98071]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
作者单位	1.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing, Peoples R China 3.First Municipal Peoples Hosp, Guangzhou, Peoples R China 4.Chinese Acad Sci, Shanghai Acad Life Sci, Inst Biochem & Cell Biol, Shanghai, Peoples R China
推荐引用方式 GB/T 7714	Liang, Aiye,Zhou, Xiaomian,Wang, Qiuyan,et al. Interactions of dextran sulfates with granulocyte colony-stimulating factor and their effects on leukemia cells[J]. electrophoresis,2006,27(16):3195-3201.
APA	Liang, Aiye.,Zhou, Xiaomian.,Wang, Qiuyan.,Liu, Xiu.,Qin, Jianhua.,...&Lin, Bingcheng.(2006).Interactions of dextran sulfates with granulocyte colony-stimulating factor and their effects on leukemia cells.electrophoresis,27(16),3195-3201.
MLA	Liang, Aiye,et al."Interactions of dextran sulfates with granulocyte colony-stimulating factor and their effects on leukemia cells".electrophoresis 27.16(2006):3195-3201.