DNA barcoding for species identification in deep-sea clams (Mollusca: Bivalvia: Vesicomyidae)

doi:10.1080/24701394.2018.1424843

CORC > 深海科学与工程研究所 > 中国科学院深海科学与工程研究所 > 深海科学研究部 > 深海生物学研究室 > 海洋生物生态与进化研究组

	DNA barcoding for species identification in deep-sea clams (Mollusca: Bivalvia: Vesicomyidae)
	Zhang, Haibin; Liu, Jun
刊名	MITOCHONDRIAL DNA PART A
	2018
卷号	29 期号:8 页码:1165-1173
关键词	COI deep-sea clams DNA barcoding molecular taxonomy Vesicomyidae
ISSN号	2470-1394
DOI	10.1080/24701394.2018.1424843
英文摘要	Deep-sea clams (Bivalvia: Vesicomyidae) have been found in reduced environments over the world oceans, but taxonomy of this group remains confusing at species and supraspecific levels due to their high-morphological similarity and plasticity. In the present study, we collected mitochondrial COI sequences to evaluate the utility of DNA barcoding on identifying vesicomyid species. COI dataset identified 56 well-supported putative species/operational taxonomic units (OTUs), approximately covering half of the extant vesicomyid species. One species (OTU2) was first detected, and may represent a new species. Average distances between species ranged from 1.65 to 29.64%, generally higher than average intraspecific distances (0-1.41%) when excluding Pliocardia sp.10 cf. venusta (average intraspecific distance 1.91%). Local barcoding gap existed in 33 of the 35 species when comparing distances of maximum interspecific and minimum interspecific distances with two exceptions (Abyssogena southwardae and Calyptogena rectimargo-starobogatovi). The barcode index number (BIN) system determined 41 of the 56 species/OTUs, each with a unique BIN, indicating their validity. Three species were found to have two BINs, together with their high level of intraspecific variation, implying cryptic diversity within them. Although fewer 16S sequences were collected, similar results were obtained. Nineteen putative species were determined and no overlap observed between intra- and inter-specific variation. Implications of DNA barcoding for the Vesicomyidae taxonomy were then discussed. Findings of this study will provide important evidence for taxonomic revision in this problematic clam group, and accelerate the discovery of new vesicomyid species in the future.
资助项目	National Natural Science Foundation of China[41576127] ; Strategic Priority Research Program of the Chinese Academy of Sciences [CAS][XDB06010104] ; Knowledge Innovation Program of CAS[SIDSSE-201401] ; Hundred Talents Program of CAS[SIDSSE-BR-201401] ; Major scientific and technological projects of Hainan Province, China[ZDKJ2016009]
WOS关键词	16S RIBOSOMAL-RNA ; OXIDASE SUBUNIT-I ; PHYLOGENETIC-RELATIONSHIPS ; EVOLUTIONARY RELATIONSHIPS ; NUCLEOTIDE-SEQUENCES ; FAMILY VESICOMYIDAE ; MAXIMUM-LIKELIHOOD ; MITOCHONDRIAL-DNA ; HYDROTHERMAL VENT ; GENE
WOS研究方向	Genetics & Heredity
语种	英语
出版者	TAYLOR & FRANCIS LTD
WOS记录号	WOS:000450275000004
资助机构	National Natural Science Foundation of China ; Strategic Priority Research Program of the Chinese Academy of Sciences [CAS] ; Knowledge Innovation Program of CAS ; Hundred Talents Program of CAS ; Major scientific and technological projects of Hainan Province, China
内容类型	期刊论文
源URL	[http://ir.idsse.ac.cn/handle/183446/5941]
专题	深海科学研究部_深海生物学研究室_海洋生物生态与进化研究组
通讯作者	Zhang, Haibin
作者单位	Chinese Acad Sci, Inst Deep Sea Sci & Engn, Sanya 57200, Peoples R China
推荐引用方式 GB/T 7714	Zhang, Haibin,Liu, Jun. DNA barcoding for species identification in deep-sea clams (Mollusca: Bivalvia: Vesicomyidae)[J]. MITOCHONDRIAL DNA PART A,2018,29(8):1165-1173.
APA	Zhang, Haibin,&Liu, Jun.(2018).DNA barcoding for species identification in deep-sea clams (Mollusca: Bivalvia: Vesicomyidae).MITOCHONDRIAL DNA PART A,29(8),1165-1173.
MLA	Zhang, Haibin,et al."DNA barcoding for species identification in deep-sea clams (Mollusca: Bivalvia: Vesicomyidae)".MITOCHONDRIAL DNA PART A 29.8(2018):1165-1173.