A Novel omega 3-Desaturase in the Deep Sea Giant Tubeworm Riftia pachyptila

doi:10.1007/s10126-017-9753-9

CORC > 深海科学与工程研究所 > 中国科学院深海科学与工程研究所 > 深海科学研究部 > 深海生物学研究室 > 海洋生物生态与进化研究组

	A Novel omega 3-Desaturase in the Deep Sea Giant Tubeworm Riftia pachyptila
	Liu, Helu; Zhang, Haibin(Chinese Acad Sci, Inst Deep Sea Sci & Engn, Sanya 572000, Peoples R China); Zhang HB(张海滨); Zhang, Haibin; Cai, Shanya; Wang, Hui
刊名	MARINE BIOTECHNOLOGY
	2017-08-01
卷号	19 期号:4 页码:345-350
关键词	Giant Tubeworm Riftia Pachyptila Pufa Biosynthesis Fatty Acid Deep Sea Hydrothermal Vent
DOI	10.1007/s10126-017-9753-9
产权排序	第1完成单位 ; 第1完成单位
文献子类	Article
英文摘要	One paradox of the trophic biochemistry of the deep sea giant tubeworm Riftia pachyptila, endemic to hydrothermal vent sites and nourished by polyunsaturated fatty acid (PUFA) deficiency chemolitoautotrophic sulfide-oxidizing bacteria, is the source of their PUFAs. Biosynthesis of PUFA starts with two precursors C18:2n-6 and C18:3n-3, which cannot be biosynthesized by most animals due to lack of omega 6- and omega 3-desaturase; thus, C18:2n-6 and C18:3n-3 are generally essential fatty acids for animals. Here, we characterized a gene derived from the R. pachyptila located by hydrothermal vent, which encoded a novel omega 3-desaturase (Rp3Fad). The gene was identified by searching the R. pachyptila transcriptome database using known omega 3-desaturases, and its predicted protein showed 37-45% identical to omega 3-desaturases of fungus and microalgae, and only 31% identitical to nematode Caenorhabditis elegans omega 3-desaturase. Expression in yeast Saccharomyces cerevisiae showed that the Rp3Fad could desaturate C18:2n-6 and C18:3n-6 into C18:3n-3 and C18:4n-3, respectively, displaying a Delta 15 activity similar to plant omega 3-desaturase, but it showed no activity towards C20 n-6 PUFA substrates, differing from the well-characterized C. elegans omega 3-desaturases Delta 5, Delta 6, Delta 8, and Delta 12 activity were also tested, resulting in no corresponding production. The function of omega 3-desaturase identified in R. pachyptila could produce C18:3n - 3 used in synthesis of n - 3 series PUFAs, suggesting an adaption to PUFA deficiency environment in deep sea hydrothermal vent.
学科主题	分子生物学
URL标识	查看原文 ; 查看原文 ; 查看原文
WOS关键词	POLYUNSATURATED FATTY-ACIDS ; MULTI-HEMOGLOBIN SYSTEM ; OMEGA-3-FATTY-ACID DESATURASE ; CAENORHABDITIS-ELEGANS ; LIPID BIOMARKERS ; BIOSYNTHESIS ; BACTERIA ; MECHANISMS ; SYMBIONTS ; BIVALVES
语种	英语
WOS记录号	WOS:000406962700003
内容类型	期刊论文
版本	出版稿
源URL	[http://ir.idsse.ac.cn/handle/183446/2600]
专题	深海科学研究部_深海生物学研究室_海洋生物生态与进化研究组
通讯作者	Zhang, Haibin(Chinese Acad Sci, Inst Deep Sea Sci & Engn, Sanya 572000, Peoples R China); Zhang HB(张海滨); Zhang, Haibin
作者单位	Chinese Acad Sci, Inst Deep Sea Sci & Engn, Sanya 572000, Peoples R China
推荐引用方式 GB/T 7714	Liu, Helu,Zhang, Haibin,Zhang HB,et al. A Novel omega 3-Desaturase in the Deep Sea Giant Tubeworm Riftia pachyptila[J]. MARINE BIOTECHNOLOGY,2017,19(4):345-350.
APA	Liu, Helu,Zhang, Haibin,张海滨,Zhang, Haibin,Cai, Shanya,&Wang, Hui.(2017).A Novel omega 3-Desaturase in the Deep Sea Giant Tubeworm Riftia pachyptila.MARINE BIOTECHNOLOGY,19(4),345-350.
MLA	Liu, Helu,et al."A Novel omega 3-Desaturase in the Deep Sea Giant Tubeworm Riftia pachyptila".MARINE BIOTECHNOLOGY 19.4(2017):345-350.