Unique tRNA gene profile suggests paucity of nucleotide modifications in anticodons of a deep-sea symbiotic Spiroplasma

doi:10.1093/narlgkx045

CORC > 深海科学与工程研究所 > 中国科学院深海科学与工程研究所 > 深海科学研究部 > 深海生物学研究室 > 海洋微生物宏基因组与分子进化研究组

	Unique tRNA gene profile suggests paucity of nucleotide modifications in anticodons of a deep-sea symbiotic Spiroplasma
	Zhu, Fang-Chao1 ; He, Li-Sheng1 ; Danchin, Antoine 2,3; Wang, Yong(Chinese Acad Sci, Inst Deep Sea Sci & Engn, Sanya, Hainan, Peoples R China); Danchin, Antoine(Hop La Pitie Salpetriere, Inst Cardiometab & Nutr, 47 Blvd Hop, F-75013 Paris, France); Wang, Yong1
刊名	NUCLEIC ACIDS RESEARCH
	2018-03-16
卷号	46 期号:5 页码:2197-2203
DOI	10.1093/narlgkx045
产权排序	第1完成单位 ; 第2完成单位 ; 第3完成单位
文献子类	Article
英文摘要	The position 34 of a tRNA is always modified for efficient recognition of codons and accurate integration of amino acids by the translation machinery. Here, we report genomics features of a deep-sea gut symbiotic Spiroplasma, which suggests that the organism does not require tRNA(34) anticodon modifications. In the genome, there is a novel set of tRNA genes composed of 32 species for recognition of the 20 amino acids. Among the anticodons of the tRNAs, we witnessed the presence of both U34- and C34 containing tRNAs required to decode NNR (A/G) 2:2 codons as countermeasure of probable loss of anticodon modification genes. In the tRNA fragments detected in the gut transcriptome, mismatches expected to be caused by some tRNA modifications were not shown in their alignments with the corresponding genes. However, the probable paucity of modified anticodons did not fundamentally change the codon usage pattern of the Spiroplasma. The tRNA gene profile that probably resulted from the paucity of tRNA(34) modifications was not observed in other symbionts and deep-sea bacteria, indicating that this phenomenon was an evolutionary deadend. This study provides insights on co-evolution of translation machine and tRNA genes and steric constraints of codon-anticodon interactions in deep-sea extreme environment.
WOS关键词	SYNONYMOUS CODON USAGE ; ESCHERICHIA-COLI ; RIBOSOME ; CONSEQUENCES ; TRANSLATION ; MYCOPLASMAS ; SPECIFICITY ; ADAPTATION ; EVOLUTION ; SELECTION
语种	英语
WOS记录号	WOS:000427677100011
内容类型	期刊论文
源URL	[http://ir.idsse.ac.cn/handle/183446/5927]
专题	深海科学研究部_深海生物学研究室_海洋微生物宏基因组与分子进化研究组深海科学研究部_深海生物学研究室
通讯作者	Danchin, Antoine; Wang, Yong(Chinese Acad Sci, Inst Deep Sea Sci & Engn, Sanya, Hainan, Peoples R China); Danchin, Antoine(Hop La Pitie Salpetriere, Inst Cardiometab & Nutr, 47 Blvd Hop, F-75013 Paris, France); Wang, Yong
作者单位	1.Chinese Acad Sci, Inst Deep Sea Sci & Engn, Sanya, Hainan, Peoples R China 2.Hop La Pitie Salpetriere, Inst Cardiometab & Nutr, 47 Blvd Hop, F-75013 Paris, France 3.Univ Hong Kong, Sch Biomed Sci, Li Kashing Fac Med, 21 Sassoon Rd, Hong Kong, Hong Kong, Peoples R China
推荐引用方式 GB/T 7714	Zhu, Fang-Chao,He, Li-Sheng,Danchin, Antoine,et al. Unique tRNA gene profile suggests paucity of nucleotide modifications in anticodons of a deep-sea symbiotic Spiroplasma[J]. NUCLEIC ACIDS RESEARCH,2018,46(5):2197-2203.
APA	Zhu, Fang-Chao,He, Li-Sheng,Danchin, Antoine,Wang, Yong,Danchin, Antoine,&Wang, Yong.(2018).Unique tRNA gene profile suggests paucity of nucleotide modifications in anticodons of a deep-sea symbiotic Spiroplasma.NUCLEIC ACIDS RESEARCH,46(5),2197-2203.
MLA	Zhu, Fang-Chao,et al."Unique tRNA gene profile suggests paucity of nucleotide modifications in anticodons of a deep-sea symbiotic Spiroplasma".NUCLEIC ACIDS RESEARCH 46.5(2018):2197-2203.