Pyrosequencing revealed shifts of prokaryotic communities between healthy and disease-like tissues of the Red Sea sponge Crella cyathophora

doi:10.7717/peerj.890

CORC > 深海科学与工程研究所 > 中国科学院深海科学与工程研究所 > 深海科学研究部 > 深海生物学研究室 > 海洋微生物宏基因组与分子进化研究组

	Pyrosequencing revealed shifts of prokaryotic communities between healthy and disease-like tissues of the Red Sea sponge Crella cyathophora
	Lafi, Feras F.1; Gao, Zhao-Ming2,3 ; Wang, Yong2,3 ; Tian, Ren-Mao 2; Lee, On On 2; Wong, Yue Him 2; Batang, Zenon B.4; Al-Suwailem, Abdulaziz 4; Bajic, Vladimir B.1; Qian, Pei-Yuan 2
刊名	PEERJ
	2015-06-11
卷号	3 期号:NO
关键词	Low Microbial Abundance Verrucomicrobia Sponge Symbiont Disease-like Sponge
DOI	10.7717/peerj.890
文献子类	Article
英文摘要	Sponge diseases have been widely reported, yet the causal factors and major pathogenic microbes remain elusive. In this study, two individuals of the sponge Crella cyathophora in total that showed similar disease-like characteristics were collected from two different locations along the Red Sea coast separated by more than 30 kilometers. The disease-like parts of the two individuals were both covered by green surfaces, and the body size was much smaller compared with adjacent healthy regions. Here, using high-throughput pyrosequencing technology, we investigated the prokaryotic communities in healthy and disease-like sponge tissues as well as adjacent seawater. Microbes in healthy tissues belonged mainly to the Proteobacteria, Cyanobacteria and Bacteroidetes, and were much more diverse at the phylum level than reported previously. Interestingly, the disease-like tissues from the two sponge individuals underwent shifts of prokaryotic communities and were both enriched with a novel clade affiliated with the phylum Verrucomicrobia, implying its intimate connection with the disease-like Red Sea sponge C. cyathophora. Enrichment of the phylum Verrucomicrobia was also considered to be correlated with the presence of algae assemblages forming the green surface of the disease-like sponge tissues. This finding represents an interesting case of sponge disease and is valuable for further study.
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WOS关键词	MICROBIAL ABUNDANCE SPONGES ; BARRIER-REEF SPONGE ; RHOPALOEIDES-ODORABILE ; MARINE SPONGES ; SEQUENCES ; EVOLUTION ; ALIGNMENT ; BACTERIA
语种	英语
WOS记录号	WOS:000356351100001
内容类型	期刊论文
版本	出版稿
源URL	[http://124.16.218.2:8080/handle/183446/500]
专题	深海科学研究部_深海生物学研究室_海洋微生物宏基因组与分子进化研究组
作者单位	1.King Abdullah Univ Sci & Technol, CBRC, Thuwal, Saudi Arabia 2.Hong Kong Univ Sci & Technol, Div Life Sci, Hong Kong, Hong Kong, Peoples R China 3.Chinese Acad Sci, Sanya Inst Deep Sea Sci & Engn, Sanya, Hai Nan, Peoples R China 4.King Abdullah Univ Sci & Technol, Coastal & Marine Resources Core Lab, Thuwal, Saudi Arabia
推荐引用方式 GB/T 7714	Lafi, Feras F.,Gao, Zhao-Ming,Wang, Yong,et al. Pyrosequencing revealed shifts of prokaryotic communities between healthy and disease-like tissues of the Red Sea sponge Crella cyathophora[J]. PEERJ,2015,3(NO).
APA	Lafi, Feras F..,Gao, Zhao-Ming.,Wang, Yong.,Tian, Ren-Mao.,Lee, On On.,...&Qian, Pei-Yuan.(2015).Pyrosequencing revealed shifts of prokaryotic communities between healthy and disease-like tissues of the Red Sea sponge Crella cyathophora.PEERJ,3(NO).
MLA	Lafi, Feras F.,et al."Pyrosequencing revealed shifts of prokaryotic communities between healthy and disease-like tissues of the Red Sea sponge Crella cyathophora".PEERJ 3.NO(2015).