Chemical Component and Proteomic Study of the Amphibalanus (= Balanus) amphitrite Shell
Qian, Pei-yuan1,2; Zhang, Yu4; Zhang, Gen1,2; Wong, Yue-Him1,2; Xu, Ying4; He, Li-sheng3
刊名PLOS ONE
2015-07-29
卷号10期号:7
DOI10.1371/journal.pone.0133866
文献子类Article
英文摘要As typical biofoulers, barnacles possess hard shells and cause serious biofouling problems. In this study, we analyzed the protein component of the barnacle Amphibalanus (= Balanus) amphitrite shell using gel-based proteomics. The results revealed 52 proteins in the A. Amphitrite shell. Among them, 40 proteins were categorized into 11 functional groups based on KOG database, and the remaining 12 proteins were unknown. Besides the known proteins in barnacle shell (SIPC, carbonic anhydrase and acidic acid matrix protein), we also identified chorion peroxidase, C-type lectin-like domains, serine proteases and proteinase inhibitor proteins in the A. Amphitrite shell. The sequences of these proteins were characterized and their potential functions were discussed. Histology and DAPI staining revealed living cells in the shell, which might secrete the shell proteins identified in this study.
WOS关键词PROTEIN COMPLEX SIPC ; C-TYPE-LECTIN ; WHEY ACIDIC PROTEIN ; CALCIUM-CARBONATE ; BARNACLE CEMENT ; ACORN BARNACLE ; MATRIX PROTEIN ; OYSTER-SHELL ; NACRE ; PURIFICATION
语种英语
WOS记录号WOS:000358836800073
内容类型期刊论文
源URL[http://ir.idsse.ac.cn/handle/183446/1212]  
专题深海科学研究部_深海生物学研究室_深海生物蛋白质组学及分子生物学研究组
作者单位1.Hong Kong Univ Sci & Technol, Sch Sci, Environm Sci Programs, Kowloon, Hong Kong, Peoples R China
2.Hong Kong Univ Sci & Technol, Sch Sci, Div Life Sci, Kowloon, Hong Kong, Peoples R China
3.Chinese Acad Sci, Inst Deep Sea Sci & Engn, Sanya City 572000, Hainan Province, Peoples R China
4.Shenzhen Univ, Coll Life Sci, Shenzhen Key Lab Marine Bioresource & Ecoenvironm, Shenzhen 518060, Peoples R China
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Qian, Pei-yuan,Zhang, Yu,Zhang, Gen,et al. Chemical Component and Proteomic Study of the Amphibalanus (= Balanus) amphitrite Shell[J]. PLOS ONE,2015,10(7).
APA Qian, Pei-yuan,Zhang, Yu,Zhang, Gen,Wong, Yue-Him,Xu, Ying,&He, Li-sheng.(2015).Chemical Component and Proteomic Study of the Amphibalanus (= Balanus) amphitrite Shell.PLOS ONE,10(7).
MLA Qian, Pei-yuan,et al."Chemical Component and Proteomic Study of the Amphibalanus (= Balanus) amphitrite Shell".PLOS ONE 10.7(2015).
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