Nitrification of archaeal ammonia oxidizers in a high-temperature hot spring

doi:10.5194/bg-13-2051-2016

CORC > 深海科学与工程研究所 > 中国科学院深海科学与工程研究所 > 深海科学研究部 > 深海地质与地球化学研究室

	Nitrification of archaeal ammonia oxidizers in a high-temperature hot spring
	Chen, Shun; Xu, Hengchao; Ta, Kaiwen; Peng, Xiaotong
刊名	BIOGEOSCIENCES
	2016
卷号	13 期号:7 页码:2051-2060
DOI	10.5194/bg-13-2051-2016
文献子类	Article
英文摘要	The oxidation of ammonia by microbes has been shown to occur in diverse natural environments. However, the link of in situ nitrification activity to taxonomic identities of ammonia oxidizers in high-temperature environments remains poorly understood. Here, we studied in situ ammonia oxidation rates and the diversity of ammonia-oxidizing Archaea (AOA) in surface and bottom sediments at 77 degrees C in the Gongxiaoshe hot spring, Tengchong, Yunnan, China. The in situ ammonia oxidation rates measured by the N-15-NO3- pool dilution technique in the surface and bottom sediments were 4.80 and 5.30 nmol N g(-1) h(-1), respectively. Real-time quantitative polymerase chain reaction (qPCR) indicated that the archaeal 16S rRNA genes and amoA genes were present in the range of 0.128 to 1.96 x 10(8) and 2.75 to 9.80 x 10(5) gene copies g(-1) sediment, respectively, while bacterial amoA was not detected. Phylogenetic analysis of 16S rRNA genes showed high sequence similarity to thermophilic Candidatus Nitrosocaldus yellowstonii, which represented the most abundant operational taxonomic units (OTU) in both surface and bottom sediments. The archaeal predominance was further supported by fluorescence in situ hybridization (FISH) visualization. The cell-specific rate of ammonia oxidation was estimated to range from 0.410 to 0.790 fmolNarchaeal cell(-1) h(-1), higher than those in the two US Great Basin hot springs. These results suggest the importance of archaeal rather than bacterial ammonia oxidation in driving the nitrogen cycle in terrestrial geothermal environments.
WOS关键词	16S RIBOSOMAL-RNA ; NITROGEN-CYCLE ; AMOA GENES ; AGRICULTURAL SOIL ; IMPORTANT PLAYERS ; YUNNAN PROVINCE ; OXIDATION ; BACTERIA ; CRENARCHAEOTA ; ABUNDANCE
语种	英语
WOS记录号	WOS:000377276000005
内容类型	期刊论文
源URL	[http://ir.idsse.ac.cn/handle/183446/1573]
专题	深海科学研究部_深海地质与地球化学研究室专家咨询委员会
作者单位	Chinese Acad Sci, Inst Deep Sea Sci & Engn, Sanya 572000, Peoples R China
推荐引用方式 GB/T 7714	Chen, Shun,Xu, Hengchao,Ta, Kaiwen,et al. Nitrification of archaeal ammonia oxidizers in a high-temperature hot spring[J]. BIOGEOSCIENCES,2016,13(7):2051-2060.
APA	Chen, Shun,Xu, Hengchao,Ta, Kaiwen,&Peng, Xiaotong.(2016).Nitrification of archaeal ammonia oxidizers in a high-temperature hot spring.BIOGEOSCIENCES,13(7),2051-2060.
MLA	Chen, Shun,et al."Nitrification of archaeal ammonia oxidizers in a high-temperature hot spring".BIOGEOSCIENCES 13.7(2016):2051-2060.