Trail armed oncolytic poxvirus suppresses lung cancer cell by inducing apoptosis | |
Hu, Jinqing1; Liu, Xinyuan1,2![]() | |
刊名 | ACTA BIOCHIMICA ET BIOPHYSICA SINICA
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2018 | |
卷号 | 50期号:10页码:1018-1027 |
关键词 | Laherparepvec T-vec Death Receptors Interacting Protein Drug-resistance Decoy Receptors Ligand Trail Expression Necrosis Gene Adenocarcinoma |
ISSN号 | 1672-9145 |
DOI | 10.1093/abbs/gmy096 |
文献子类 | Article |
英文摘要 | Lung cancer has a high morbidity rate worldwide and is often resistant to therapy. Oncolytic virus therapy is a developing trend for cancer treatment. Thus, we constructed an oncolytic poxvirus carrying human trail gene that expresses a membrane-binding tumor necrosis factor and associated apoptosis-inducing ligand (TRAIL, Oncopox-trail). We hypothesized that the expression of trail would increase the efficacy of the oncolytic poxvirus. The effect of the TRAIL protein depends on the death receptors on the surface of different cancer cells. The expression of death receptors in lung cancer cell lines was analyzed by western blot analysis. In vitro, the oncolytic poxvirus carrying the trail gene displayed a better cytotoxicity at the cell level in the lung cancer cell line than that carrying the Oncopox-empty. TRAIL protein mainly induced apoptosis and inhibited necrosis. In vivo, two transplanted tumor models of human A549 lung cancer cells and mouse Lewis lung cancer cells were used to verify the anti-cancer effect of the oncolytic poxvirus carrying the trail gene. TUNEL staining results of the tumor histological sections also verified the anti-cancer effect. Similarly, through systemic administration of Oncopox-trail, the oncolytic poxvirus also exhibited anti-cancer effect. |
电子版国际标准刊号 | 1745-7270 |
WOS研究方向 | Biochemistry & Molecular Biology ; Biophysics |
语种 | 英语 |
WOS记录号 | WOS:000448771600009 |
内容类型 | 期刊论文 |
版本 | 出版稿 |
源URL | [http://202.127.25.143/handle/331003/3507] ![]() |
专题 | 生化所2018年发文 上海生化细胞研究所_上海生科院生化细胞研究所 |
通讯作者 | Liu, Xinyuan; Zhou, Xiumei |
作者单位 | 1.Zhejiang Sci Tech Univ, Sch Life Sci, Xinyuan Inst Med & Biotechnol, Lab Cell Biol, Hangzhou 310018, Zhejiang, Peoples R China; 2.Chinese Acad Sci, CAS Ctr Excellence Mol Cell Sci, Innovat Ctr Cell Signaling Network,Shanghai Inst, State Key Lab Cell Biol,Inst Biochem & Cell Biol, Shanghai 200031, Peoples R China |
推荐引用方式 GB/T 7714 | Hu, Jinqing,Liu, Xinyuan,Zhou, Xiumei,et al. Trail armed oncolytic poxvirus suppresses lung cancer cell by inducing apoptosis[J]. ACTA BIOCHIMICA ET BIOPHYSICA SINICA,2018,50(10):1018-1027. |
APA | Hu, Jinqing,Liu, Xinyuan,Zhou, Xiumei,Wang, Huaiyuan,&Gu, Jinfa.(2018).Trail armed oncolytic poxvirus suppresses lung cancer cell by inducing apoptosis.ACTA BIOCHIMICA ET BIOPHYSICA SINICA,50(10),1018-1027. |
MLA | Hu, Jinqing,et al."Trail armed oncolytic poxvirus suppresses lung cancer cell by inducing apoptosis".ACTA BIOCHIMICA ET BIOPHYSICA SINICA 50.10(2018):1018-1027. |
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