Efficient vector systems for economical and rapid epitope-tagging and overexpression in Candida albicans

doi:10.1016/j.mimet.2018.04.016

CORC > 上海生物化学与细胞生物学研究所 > 上海生科院生化细胞研究所 > 生化所2018年发文

	Efficient vector systems for economical and rapid epitope-tagging and overexpression in Candida albicans
	Chang, Peng 1; Igarashi, Yasuo 1; Luo, Feng 1; Wang, Wenjuan 2; Chen, Jiangye2
刊名	JOURNAL OF MICROBIOLOGICAL METHODS
	2018
卷号	149 页码:14-19
关键词	HYPHAL DEVELOPMENT TRANSCRIPTION FACTOR GENE MANIPULATION GLOBAL ANALYSIS EXPRESSION VIRULENCE CASSETTES LOCALIZATION CONSTRUCTION DISRUPTION
ISSN号	0167-7012
DOI	10.1016/j.mimet.2018.04.016
文献子类	Article
英文摘要	Candida albicans is an opportunistic pathogenic fungus which causes superficial and systemic infections in immunocompromised patients. It is important to characterize the roles of genes involved in its pathogenesis, virulence, and drug resistance. Several genetic manipulation toolkits have been developed for gene function research in C. albicans. Here, we describe efficient vector systems that allow economical and rapid C-terminal and N-terminal epitope-tagging, inducible and constitutive promoter replacements, and ectopic gene over expression in C. albicans. These systems use modularized genetic elements (conventional and non-conventional selection markers, epitope tags and promoters) and universal primers. These advantages should greatly reduce laboratory work and costs of strain construction for C. albicans.
电子版国际标准刊号	1872-8359
WOS研究方向	Biochemical Research Methods ; Microbiology
语种	英语
WOS记录号	WOS:000435063300003
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/3399]
专题	生化所2018年发文
作者单位	1.Southwest Univ, Coll Resources & Environm, Res Ctr Bioenergy & Bioremediat, Chongqing 400715, Peoples R China; 2.Chinese Acad Sci, Shanghai Inst Biochem & Cell Biol, Shanghai 200031, Peoples R China
推荐引用方式 GB/T 7714	Chang, Peng,Igarashi, Yasuo,Luo, Feng,et al. Efficient vector systems for economical and rapid epitope-tagging and overexpression in Candida albicans[J]. JOURNAL OF MICROBIOLOGICAL METHODS,2018,149:14-19.
APA	Chang, Peng,Igarashi, Yasuo,Luo, Feng,Wang, Wenjuan,&Chen, Jiangye.(2018).Efficient vector systems for economical and rapid epitope-tagging and overexpression in Candida albicans.JOURNAL OF MICROBIOLOGICAL METHODS,149,14-19.
MLA	Chang, Peng,et al."Efficient vector systems for economical and rapid epitope-tagging and overexpression in Candida albicans".JOURNAL OF MICROBIOLOGICAL METHODS 149(2018):14-19.