Comparative metabolic profiling of Vitis amurensis and Vitis vinifera during cold acclimation

doi:10.1038/s41438-018-0083-5

CORC > 武汉植物园 > 中国科学院武汉植物园

	Comparative metabolic profiling of Vitis amurensis and Vitis vinifera during cold acclimation
	Li, Shaohua 5; Xin, Haiping 6; Duan, Lixin 1; Liu, Guotian 2; Cheng, Cheng 4,6; Sun, Xiaoming 5,6; Meng, Xianbin 3; Xiang, Yue 4; Liu, Wenwen 4,5; Chai, Fengmei 4,5,6
刊名	HORTICULTURE RESEARCH
	2019
卷号	6 页码:12
ISSN号	2052-7276
DOI	10.1038/s41438-018-0083-5
英文摘要	Vitis amurensis is a wild Vitis plant that can withstand extreme cold temperatures. However, the accumulation of metabolites during cold acclimation (CA) in V. amurensis remains largely unknown. In this study, plantlets of V amurensis and V vinifera cv. Muscat of Hamburg were treated at 4 degrees C for 24 and 72 h, and changes of metabolites in leaves were detected by gas chromatography coupled with time-of-flight mass spectrometry. Most of the identified metabolites, including carbohydrates, amino acids, and organic acids, accumulated in the two types of grape after CA. Galactinol, raffinose, fructose, mannose, glycine, and ascorbate were continuously induced by cold in V amurensis, but not in Muscat of Hamburg. Twelve metabolites, including isoleucine, valine, proline, 2-oxoglutarate, and putrescine, increased in V amurensis during CA. More galactinol, ascorbate, 2-oxoglutarate, and putrescine, accumulated in V amurensis, but not in Muscat of Hamburg, during CA, which may be responsible for the excellent cold tolerance in V amurensis. The expression levels of the genes encoding (3-amylase (BAMY), galactinol synthase (GoIS), and raffinose synthase (RafS) were evaluated by quantitative reverse transcription-PCR. The expression BAMY (VIT_02s0012 g00170) and RafS (VIT_05s0077 g00840) were primarily responsible for the accumulation of maltose and raffinose, respectively. The accumulation of galactinol was attributed to different members of GoIS in the two grapes. In conclusion, these results show the inherent differences in metabolites between V amurensis and V. vinifera under CA.
资助项目	Youth Innovation Promotion Association of CAS[2015281] ; National Natural Science Foundation of China[31471857] ; National Natural Science Foundation of China[31672132] ; Science and Technology Service Network Initiative of CAS[KFJ-STS-ZDTP-025] ; Grape Breeding Project of Ningxia[NXNYYZ201502]
WOS研究方向	Plant Sciences ; Agriculture
语种	英语
出版者	NATURE PUBLISHING GROUP
WOS记录号	WOS:000454602600008
内容类型	期刊论文
源URL	[http://202.127.146.157/handle/2RYDP1HH/6417]
专题	中国科学院武汉植物园
通讯作者	Li, Shaohua; Xin, Haiping
作者单位	1.Guangzhou Univ Chinese Med, Int Inst Translat Chinese Med, Guangzhou, Guangdong, Peoples R China 2.Northwest A&F Univ, Coll Hort, State Key Lab Crop Stress Biol Arid Areas, Yangling, Shaanxi, Peoples R China 3.Chinese Acad Sci, Inst Bot, Key Lab Plant Mol Physiol, Beijing, Peoples R China 4.Univ Chinese Acad Sci, Beijing, Peoples R China 5.Chinese Acad Sci, Inst Bot, CAS Key Lab Plant Resources, Beijing Key Lab Grape Sci & Enol, Beijing, Peoples R China 6.Chinese Acad Sci, Key Lab Plant Germplasm Enhancement & Specialty A, Wuhan Bot Garden, Wuhan, Hubei, Peoples R China
推荐引用方式 GB/T 7714	Li, Shaohua,Xin, Haiping,Duan, Lixin,et al. Comparative metabolic profiling of Vitis amurensis and Vitis vinifera during cold acclimation[J]. HORTICULTURE RESEARCH,2019,6:12.
APA	Li, Shaohua.,Xin, Haiping.,Duan, Lixin.,Liu, Guotian.,Cheng, Cheng.,...&Chai, Fengmei.(2019).Comparative metabolic profiling of Vitis amurensis and Vitis vinifera during cold acclimation.HORTICULTURE RESEARCH,6,12.
MLA	Li, Shaohua,et al."Comparative metabolic profiling of Vitis amurensis and Vitis vinifera during cold acclimation".HORTICULTURE RESEARCH 6(2019):12.