DNA barcoding of Actinidia (Actinidiaceae) using internal transcribed spacer, matK, rbcL and trnH-psbA, and its taxonomic implication

doi:10.1080/0028825X.2018.1491009

CORC > 武汉植物园 > 中国科学院武汉植物园

	DNA barcoding of Actinidia (Actinidiaceae) using internal transcribed spacer, matK, rbcL and trnH-psbA, and its taxonomic implication
	Li, Xinwei 2; Li, Dawei 2; Bao Weihong 1,2
刊名	NEW ZEALAND JOURNAL OF BOTANY
	2018
卷号	56 期号:4 页码:360-371
关键词	Actinidia DNA barcoding ITS matK rbcL trnH-psbA
ISSN号	0028-825X
DOI	10.1080/0028825X.2018.1491009
英文摘要	Actinidia is taxonomically difficult and economically important. Four traditional barcoding markers, internal transcribed spacer (ITS), rbcL, matK and trnH-psbA, were used to identify the 29 Actinidia species sampled. High-quality sequences could be obtained easily for rbcL, matK and trnH-psbA, and matK performed best at resolving species among these three markers. ITS had a moderate sequencing success of 72% and the species resolution proportion was 60.7%. Sequencing success rate for matK+rbcL was 97.4% and it discriminated 48.3% of the species analysed. The barcode trnH-psbA could only identify Actinidia eriantha. MatK+rbcL and ITS are useful markers to barcode Actinidia; the utility of ITS in barcoding needs further investigation using high-throughput sequencing technology. Phylogenetic analyses based on ITS, matK, matK+rbcL and matK+rbcL+trnH-psbA indicated Sect. Leiocarpae to be paraphyletic, while Sect. Maculatae and Sect. Strigosae together with Sect. Stellatae formed a monophyletic group. We recommended the subdivision of Actinidia into two groups: one consisting of Sect. Leiocarpae (ovaries glabrous, fruits spotless), and the other comprising sections Maculatae, Strigosae and Stellatae (ovaries hairy, fruits spotted). This study supported the separation of Actinidia chinensis var. chinensis and var. deliciosa at the infraspecific level, and the separation of Actinidia tetramera and Actinidia kolomikta at the specific level. The treatment of Actinidia maloides as a synonym of A.kolomikta and Actinidia cinerascens as a variety of Actinidia fulvicoma was also warranted.
WOS研究方向	Plant Sciences
语种	英语
出版者	TAYLOR & FRANCIS LTD
WOS记录号	WOS:000452052000002
内容类型	期刊论文
源URL	[http://202.127.146.157/handle/2RYDP1HH/6365]
专题	中国科学院武汉植物园
通讯作者	Li, Xinwei
作者单位	1.Univ Chinese Acad Sci, Coll Life Sci, Beijing, Peoples R China 2.Chinese Acad Sci, Wuhan Bot Garden, Key Lab Plant Germplasm Enhancement & Specialty A, Wuhan, Hubei, Peoples R China
推荐引用方式 GB/T 7714	Li, Xinwei,Li, Dawei,Bao Weihong. DNA barcoding of Actinidia (Actinidiaceae) using internal transcribed spacer, matK, rbcL and trnH-psbA, and its taxonomic implication[J]. NEW ZEALAND JOURNAL OF BOTANY,2018,56(4):360-371.
APA	Li, Xinwei,Li, Dawei,&Bao Weihong.(2018).DNA barcoding of Actinidia (Actinidiaceae) using internal transcribed spacer, matK, rbcL and trnH-psbA, and its taxonomic implication.NEW ZEALAND JOURNAL OF BOTANY,56(4),360-371.
MLA	Li, Xinwei,et al."DNA barcoding of Actinidia (Actinidiaceae) using internal transcribed spacer, matK, rbcL and trnH-psbA, and its taxonomic implication".NEW ZEALAND JOURNAL OF BOTANY 56.4(2018):360-371.