Covalently Assembled Dipeptide Nanoparticles with Adjustable Fluorescence Emission for Multicolor Bioimaging

doi:10.1002/cbic.201800434

	Covalently Assembled Dipeptide Nanoparticles with Adjustable Fluorescence Emission for Multicolor Bioimaging
	Li, Shukun 1; Liu, Yamei 1; Xing, Ruirui 1; Yan, Xuehai 1,2
刊名	CHEMBIOCHEM
	2019-02-15
卷号	20 期号:4 页码:555-560
关键词	fluorescence imaging agents nanoparticles peptides self-assembly
ISSN号	1439-4227
DOI	10.1002/cbic.201800434
英文摘要	Peptide self-assembly, inspired by the naturally occurring fabrication principle, remains the most attractive in constructing fluorescent nanoagents towards bioimaging. However, the noncovalent interactions that drive peptide self-assembly are usually susceptible to the complex physiological environment; thus leading to disassembly and dysfunction of fluorescent nanoagents. Herein, a covalently crosslinked assembly strategy for fabrication of stable peptide-based nanoparticles with adjustable emission is introduced. In the process of cationic diphenylalanine peptide (H-Phe-Phe-NH2 center dot HCl) self-assembly, glutaraldehyde is used as a crosslinker and the resulting product of the Schiff base reaction can be fluorescent. More importantly, the emission wavelength can be readily tuned by controlling the covalent reaction time. It has been demonstrated that the nanoparticles are stable, even after intracellular uptake, and can be used for sustainable multicolor fluorescent imaging. The strategy with integrating peptide self-assembly and covalent crosslinking could be promising for the design and engineering of functional fluorescent nanoparticles with robust physiological stability and adjustable emission towards improved bioimaging applications.
资助项目	National Natural Science Foundation of China[21522307] ; National Natural Science Foundation of China[21473208] ; National Natural Science Foundation of China[201773248] ; Talent Fund of the Recruitment Program of Global Youth Experts ; Key Research Program of the Frontier Sciences of the Chinese Academy of Sciences[QYZDB-SSW-JSC034] ; CAS President's International Fellowship Initiative[2018VEA0005]
WOS关键词	CONJUGATED POLYMER NANOPARTICLES ; SEMICONDUCTING POLYMER ; CONSTRUCTION ; NANOCARRIERS ; DELIVERY ; DESIGN ; PROBES ; DOTS ; TIME
WOS研究方向	Biochemistry & Molecular Biology ; Pharmacology & Pharmacy
语种	英语
出版者	WILEY-V C H VERLAG GMBH
WOS记录号	WOS:000458825800016
资助机构	National Natural Science Foundation of China ; Talent Fund of the Recruitment Program of Global Youth Experts ; Key Research Program of the Frontier Sciences of the Chinese Academy of Sciences ; CAS President's International Fellowship Initiative
内容类型	期刊论文
源URL	[http://ir.ipe.ac.cn/handle/122111/28029]
专题	中国科学院过程工程研究所
通讯作者	Yan, Xuehai
作者单位	1.Chinese Acad Sci, Inst Proc Engn, State Key Lab Biochem Engn, 1 North Second St, Beijing 100190, Peoples R China 2.Chinese Acad Sci, Inst Proc Engn, Ctr Mesosci, Beijing 100190, Peoples R China
推荐引用方式 GB/T 7714	Li, Shukun,Liu, Yamei,Xing, Ruirui,et al. Covalently Assembled Dipeptide Nanoparticles with Adjustable Fluorescence Emission for Multicolor Bioimaging[J]. CHEMBIOCHEM,2019,20(4):555-560.
APA	Li, Shukun,Liu, Yamei,Xing, Ruirui,&Yan, Xuehai.(2019).Covalently Assembled Dipeptide Nanoparticles with Adjustable Fluorescence Emission for Multicolor Bioimaging.CHEMBIOCHEM,20(4),555-560.
MLA	Li, Shukun,et al."Covalently Assembled Dipeptide Nanoparticles with Adjustable Fluorescence Emission for Multicolor Bioimaging".CHEMBIOCHEM 20.4(2019):555-560.