Enhanced Cytosolic Delivery and Release of CRISPR/Cas9 by Black Phosphorus Nanosheets for Genome Editing

	Enhanced Cytosolic Delivery and Release of CRISPR/Cas9 by Black Phosphorus Nanosheets for Genome Editing
	Zhou, Wenhua; Cui, Haodong; Ying, Liming; Yu, Xue-Feng
刊名	ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
	2018
文献子类	期刊论文
英文摘要	A biodegradable two-dimensional (2D) delivery platform based on loading black phosphorus nanosheets (BPs) with Cas9 ribonucleoprotein engineered with three nuclear localization signals (NLSs) at C terminus (Cas9N3) is successfully established. The Cas9N3-BPs enter cells effectively via membrane penetration and endocytosis pathways, followed by a BPs biodegradation-associated endosomal escape and cytosolic releases of the loaded Cas9N3 complexes. The Cas9N3-BPs thus provide efficient genome editing and gene silencing invitro and invivo at a relatively low dose as compared with other nanoparticle-based delivery platforms. This biodegradable 2D delivery platform offers a versatile cytosolic delivery approach for CRISPR/Cas9 ribonucleoprotein and other bioactive macromolecules for biomedical applications.
URL标识	查看原文
语种	英语
内容类型	期刊论文
源URL	[http://ir.siat.ac.cn:8080/handle/172644/14661]
专题	深圳先进技术研究院_医药所
推荐引用方式 GB/T 7714	Zhou, Wenhua,Cui, Haodong,Ying, Liming,et al. Enhanced Cytosolic Delivery and Release of CRISPR/Cas9 by Black Phosphorus Nanosheets for Genome Editing[J]. ANGEWANDTE CHEMIE-INTERNATIONAL EDITION,2018.
APA	Zhou, Wenhua,Cui, Haodong,Ying, Liming,&Yu, Xue-Feng.(2018).Enhanced Cytosolic Delivery and Release of CRISPR/Cas9 by Black Phosphorus Nanosheets for Genome Editing.ANGEWANDTE CHEMIE-INTERNATIONAL EDITION.
MLA	Zhou, Wenhua,et al."Enhanced Cytosolic Delivery and Release of CRISPR/Cas9 by Black Phosphorus Nanosheets for Genome Editing".ANGEWANDTE CHEMIE-INTERNATIONAL EDITION (2018).