Single Microfluidic enrichment of plasma cells improves treatment of multiple myeloma

	Single Microfluidic enrichment of plasma cells improves treatment of multiple myeloma
	S. C. Li; Y. Zeng; L. Gao; X. Luo; Y. Chen; M. H. Kabeer; X. Chen; A. Stucky; W. G. Loudon; X. Zhang
刊名	Molecular Oncology
	2018
文献子类	期刊论文
英文摘要	Cytogenetic alterations form the basis for risk stratification for multiple myeloma (MM) and guide the selection of therapy; however, current pathology assays performed on bone marrow samples can produce falsenegatives due to the npredictable distribution and rarity of MM cells. Here, we report on a microfluidic device used to facilitate CD45 depletion to enhance the detection of cytogenetic alterations in plasma cells (PCs). Bone marrow samples from 48 patients with MM were each divided into two aliquots. One aliquot was subjected to classic flow cytometry and fluorescent in situ hybridization (FISH). The other first went through CD45+ cell depletion, further enriched by microfluidic size selection. The enriched samples were then analyzed using flow cytometry and FISH and compared to those analyzed using the classic method only. Unlike the traditional method, the microfluidic device removed the CD45+ leukocytes and specifically selected PCs from the remaining white blood cells. Therefore, the microfluidic method (MF-CD45-TACs) significantly increased the percentage of CD38+/CD138+ cells to 37.7 _ 20.4% (P < 0.001) from 10.3 _8.5% in bone marrow. After the MF-CD45-TAC enrichment, the detection rate of IgH rearrangement, del(13q14), del(17p), and 1q21 gains, rose to 56.3% (P < 0.001), 37.5% (P < 0.001), 22.9% (P < 0.001), and 41.7% (P = 0.001), respectively; all rates of detection were significantly increased compared to the classically analyzed samples. In this clinical trial, this microfluidic-assisted assay provided a precise detection of cytogenetic alterations in PCs and improved clinical outcomes.
URL标识	查看原文
语种	英语
内容类型	期刊论文
源URL	[http://ir.siat.ac.cn:8080/handle/172644/14301]
专题	深圳先进技术研究院_医工所
推荐引用方式 GB/T 7714	S. C. Li,Y. Zeng,L. Gao,et al. Single Microfluidic enrichment of plasma cells improves treatment of multiple myeloma[J]. Molecular Oncology,2018.
APA	S. C. Li.,Y. Zeng.,L. Gao.,X. Luo.,Y. Chen.,...&J. F. Zhong.(2018).Single Microfluidic enrichment of plasma cells improves treatment of multiple myeloma.Molecular Oncology.
MLA	S. C. Li,et al."Single Microfluidic enrichment of plasma cells improves treatment of multiple myeloma".Molecular Oncology (2018).