Three Dimensional Hessian matrix based quantitative vascular imaging of rat iris with optical-resolution photoacoustic microscopy

	Three Dimensional Hessian matrix based quantitative vascular imaging of rat iris with optical-resolution photoacoustic microscopy
	Huangxuan Zhao; Guangsong Wang; Wenjia Wang; Kunya Zhang; Xiuqing Qian; Haixia Zhang; Lin Li; Zhicheng Liu; Chengbo Liu; Riqiang Lin
刊名	Journal of Biomedical Optics
	2018
文献子类	期刊论文
英文摘要	For the diagnosis and evaluation of ophthalmic diseases, imaging and quantitative characterization of vasculature in the iris are very important. The recently developed photoacoustic imaging, which is ultrasensitive in imaging endogenous hemoglobin molecules, provides a highly efficient label-free method for imaging blood vasculature in the iris. However, the development of advanced vascular quantification algorithms is still needed to enable accurate characterization of the underlying vasculature. We have developed a vascular information quantification algorithm by adopting a three-dimensional (3-D) Hessian matrix and applied for processing iris vasculature images obtained with a custom-built optical-resolution photoacoustic imaging system (OR-PAM). For the first time, we demonstrate in vivo 3-D vascular structures of a rat iris with a the label-free imaging method and also accurately extract quantitative vascular information, such as vessel diameter, vascular density, and vascular tortuosity. Our results indicate that the developed algorithm is capable of quantifying the vasculature in the 3-D photoacoustic images of the iris in-vivo, thus enhancing the diagnostic capability of the OR-PAM system for vascular-related ophthalmic diseases in vivo.
URL标识	查看原文
语种	英语
内容类型	期刊论文
源URL	[http://ir.siat.ac.cn:8080/handle/172644/14215]
专题	深圳先进技术研究院_医工所
推荐引用方式 GB/T 7714	Huangxuan Zhao, Guangsong Wang, Wenjia Wang,et al. Three Dimensional Hessian matrix based quantitative vascular imaging of rat iris with optical-resolution photoacoustic microscopy[J]. Journal of Biomedical Optics,2018.
APA	Huangxuan Zhao., Guangsong Wang., Wenjia Wang., Kunya Zhang.,Xiuqing Qian.,...& Tan Li.(2018).Three Dimensional Hessian matrix based quantitative vascular imaging of rat iris with optical-resolution photoacoustic microscopy.Journal of Biomedical Optics.
MLA	Huangxuan Zhao,et al."Three Dimensional Hessian matrix based quantitative vascular imaging of rat iris with optical-resolution photoacoustic microscopy".Journal of Biomedical Optics (2018).