Evaluation of genotoxic effects of surface waters using a battery of bioassays indicating different mode of action

doi:10.1016/lecoenv.2016.07.022

CORC > 生态环境研究中心 > 中国科学院生态环境研究中心 > 中国科学院饮用水科学与技术重点实验室

	Evaluation of genotoxic effects of surface waters using a battery of bioassays indicating different mode of action
	Oda, Yoshimitsu; Han, Yingnan; Li, Na; Ma, Mei; Rao, Kaifeng; Wang, Zijian; Jin, Wei; Hong, Gang; Li, Zhiguo; Luo, Yi
刊名	ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
	2016-11
卷号	133 期号:0 页码:448-456
关键词	Surface Water Genotoxidty Sos/umu Test Micronucleus Assay Mode Of Action
DOI	10.1016/lecoenv.2016.07.022
英文摘要	With the burgeoning contamination of surface waters threatening human health, the genotoxic effects of surface waters have received much attention. Because mutagenic and carcinogenic compounds in water cause tumors by different mechanisms, a battery of bioassays that each indicate a different mode of action (MOA) is required to evaluate the genotoxic effects of contaminants in water samples. In this study, 15 water samples from two source water reservoirs and surrounding rivers in Shijiazhuang city of China were evaluated for genotoxic effects. Target chemical analyses of 14 genotoxic pollutants were performed according to the Environmental quality standards for surface water of China. Then, the in vitro cytokinesis-block micronucleus (CBMN) assay, based on a high -content screening technique, was used to detect the effect of chromosome damage. The SOS/umu test using strain TA1535/pSK1002 was used to detect effects on SOS repair of gene expression. Additionally, two other strains, NM2009 and NM3009, which are highly sensitive to aromatic amines and nitroarenes, respectively, were used in the SOS/umu test to avoid false negative results. In the water samples, only two of the genotoxic chemicals listed in the water standards were detected in a few samples, with concentrations that were below water quality standards. However, positive results for the CBMN assay were observed in two river samples, and positive results for the induction of umuC gene expression in TA1535/pSK1002 were observed in seven river samples. Moreover, positive results were observed for NM2009 with S9 and NM3009 without S9 in some samples that had negative results using the strain TA1535/pSK1002. Based on the results with NM2009 and NM3009, some unknown or undetected aromatic amines and nitroarenes were likely in the source water reservoirs and the surrounding rivers. Furthermore, these compounds were most likely the causative pollutants for the genotoxic effect of these water samples. Therefore, to identify causative pollutants with harmful biological effects, chemical analyses for the pollutants listed in water quality standards is not sufficient, and single -endpoint bioassays may underestimate adverse effects. Thus, a battery of bioassays based on different MOAs is required for the comprehensive detection of harmful biological effects. In conclusion, for genotoxicity screening of surface waters, the SOS/umu test system by using different strains combined with the CBMN assay was a useful approach. (C) 2016 Elsevier Inc. All rights reserved.
内容类型	期刊论文
源URL	[http://ir.rcees.ac.cn/handle/311016/36182]
专题	生态环境研究中心_中国科学院饮用水科学与技术重点实验室
推荐引用方式 GB/T 7714	Oda, Yoshimitsu,Han, Yingnan,Li, Na,et al. Evaluation of genotoxic effects of surface waters using a battery of bioassays indicating different mode of action[J]. ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY,2016,133(0):448-456.
APA	Oda, Yoshimitsu.,Han, Yingnan.,Li, Na.,Ma, Mei.,Rao, Kaifeng.,...&Luo, Yi.(2016).Evaluation of genotoxic effects of surface waters using a battery of bioassays indicating different mode of action.ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY,133(0),448-456.
MLA	Oda, Yoshimitsu,et al."Evaluation of genotoxic effects of surface waters using a battery of bioassays indicating different mode of action".ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY 133.0(2016):448-456.