Dynamic Succession of Groundwater Sulfate-Reducing Communities during Prolonged Reduction of Uranium in a Contaminated Aquifer

CORC > 生态环境研究中心 > 中国科学院生态环境研究中心 > 中国科学院环境生物技术重点实验室

	Dynamic Succession of Groundwater Sulfate-Reducing Communities during Prolonged Reduction of Uranium in a Contaminated Aquifer
	Wu, Liyou; Zhang, Ping; He, Zhili; Van Nostrand, Joy D.; Qin, Yujia; Hazen, Terry C.; Arkin, Adam P.; Stahl, David A.; Zhou, Jizhong; Fields, Matthew W.
刊名	ENVIRONMENTAL SCIENCE & TECHNOLOGY
	2017-04-04
卷号	51 期号:7 页码:3609-3620
英文摘要	To further understand the diversity and dynamics of SRB in response to substrate amendment, we sequenced genes coding for the dissimilatory sulfite reductase (dsrA) in groundwater samples collected after an emulsified vegetable oil (EVO) amendment, which sustained U(VI)-reducing conditions for one year in a fast-flowing aquifer. EVO amendment significantly altered the composition of groundwater SRB communities. Sequences having no closely related-described species dominated (80%) the indigenous SRB communities in nonamended wells. After EVO amendment, Desulfococcus, Desulfobacterium, and Desulfovibrio, known for long-chain-fatty-acid, short-chain-fatty-acid and H-2 oxidation and U(VI) reduction, became dominant accounting for 7 +/- 2%, 21 +/- 8%, and 55 +/- 8% of the SRB communities, respectively. Succession of these SRB at different bioactivity stages based on redox substrates/products (acetate, SO4-2, U(VI), NO3-, Fe(II), and Mn(II)) was observed. Desulfovibrio and Desulfococcus dominated SRB communities at 4-31 days, whereas Desulfobacterium became dominant at 80-140 days. By the end of the experiment (day 269), the abundance of these SRB decreased but the overall diversity of groundwater SRB was still higher than non-EVO controls. Up to 62% of the SRB community changes could be explained by groundwater geochemical variables, including those redox substrates/products. A significant (P < 0.001) correlation was observed between groundwater U(VI) concentrations and Desulfovibrio abundance. Our results showed that the members of SRB and their dynamics were correlated significantly with slow EVO biodegradation, electron donor production and maintenance of U(VI)-reducing conditions in the aquifer.
内容类型	期刊论文
源URL	[http://ir.rcees.ac.cn/handle/311016/39621]
专题	生态环境研究中心_中国科学院环境生物技术重点实验室
推荐引用方式 GB/T 7714	Wu, Liyou,Zhang, Ping,He, Zhili,et al. Dynamic Succession of Groundwater Sulfate-Reducing Communities during Prolonged Reduction of Uranium in a Contaminated Aquifer[J]. ENVIRONMENTAL SCIENCE & TECHNOLOGY,2017,51(7):3609-3620.
APA	Wu, Liyou.,Zhang, Ping.,He, Zhili.,Van Nostrand, Joy D..,Qin, Yujia.,...&Deng, Ye.(2017).Dynamic Succession of Groundwater Sulfate-Reducing Communities during Prolonged Reduction of Uranium in a Contaminated Aquifer.ENVIRONMENTAL SCIENCE & TECHNOLOGY,51(7),3609-3620.
MLA	Wu, Liyou,et al."Dynamic Succession of Groundwater Sulfate-Reducing Communities during Prolonged Reduction of Uranium in a Contaminated Aquifer".ENVIRONMENTAL SCIENCE & TECHNOLOGY 51.7(2017):3609-3620.