Simultaneous saccharification and fermentation of hemicellulose to butanol by a non-sporulating Clostridium species

doi:10.1016/j.biortech.2016.07.138

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	Simultaneous saccharification and fermentation of hemicellulose to butanol by a non-sporulating Clostridium species
	He, Jianzhong; Li, Tinggang
刊名	BIORESOURCE TECHNOLOGY
	2016-11
卷号	219 页码:430-438
关键词	Clostridium Butanol Xylan No acetone/ethaNol Formation Carbon Catabolite Derepression
DOI	10.1016/j.biortech.2016.07.138
英文摘要	Production of lignocellulosic butanol has drawn increasing attention. However, currently few microorganisms can produce biofuels, particularly butanol, from lignocellulosic biomass via simultaneous saccharification and fermentation. Here we report discovery of a wild-type, mesophilic Clostridium sp. strain MF28 that ferments xylan to produce butanol (up to 3.2 g/L) without the addition of saccharolytic enzymes and without any chemical pretreatments. Application of selective pressure from 2-deoxy-Dglucose facilitated isolation of strain MF28, which exhibits inactivation of genes (gid and ccp genes) responsible for carbon catabolite repression, thus allowing strain MF28 to simultaneously ferment a combination of glucose (30 g/L), xylose (15 g/L), and arabinose (15 g/L) to produce 11.9 g/L of butanol. Strain MF28 possesses several unique features: (i) non-sporulating, (ii) no acetone/ethanol, (iii) complete hemicellulose-binding enzymatic domain, and (iv) absence of carbon catabolite repression. These unique characteristics demonstrate the industrial potential of strain MF28 for cost-effective biofuel generation from lignocellulosic biomass. (C) 2016 Elsevier Ltd. All rights reserved.
内容类型	期刊论文
源URL	[http://ir.rcees.ac.cn/handle/311016/36262]
专题	生态环境研究中心_土壤环境科学实验室
推荐引用方式 GB/T 7714	He, Jianzhong,Li, Tinggang. Simultaneous saccharification and fermentation of hemicellulose to butanol by a non-sporulating Clostridium species[J]. BIORESOURCE TECHNOLOGY,2016,219:430-438.
APA	He, Jianzhong,&Li, Tinggang.(2016).Simultaneous saccharification and fermentation of hemicellulose to butanol by a non-sporulating Clostridium species.BIORESOURCE TECHNOLOGY,219,430-438.
MLA	He, Jianzhong,et al."Simultaneous saccharification and fermentation of hemicellulose to butanol by a non-sporulating Clostridium species".BIORESOURCE TECHNOLOGY 219(2016):430-438.