Isolation and purification of epirubicin from raw product by preparative chromatography on a silica column with aqueous-rich mobile phase

doi:10.1080/10826070500224955

CORC > 生态环境研究中心 > 中国科学院生态环境研究中心 > 水污染控制实验室

	Isolation and purification of epirubicin from raw product by preparative chromatography on a silica column with aqueous-rich mobile phase
	Li, RP; Huang, JX
刊名	JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES
	2005
卷号	28 期号:17 页码:2737-2751
关键词	Preparative Chromatography Mobile Phase Composition Optimization Epirubicin Antibiotics
DOI	10.1080/10826070500224955
英文摘要	A novel chromatographic method has been developed for the preparative separation of epirubicin from raw product solution in overloaded elution mode using an analytical silica column with aqueous-rich mobile phase. The retention behavior of sample solutes was investigated in order to optimize the resolution by changing the mobile phase composition, flow-rate, and column temperature. It has been found that the high-purity silica exhibits typical reversed-phase behavior in a highly aqueous environment with little or organic modifier, and the nature of the organic modifier has little effect on its hydrophobic property. More significantly, the overloaded peak profiles of the solutes under such conditions were right-angled triangle with the rear of the peaks in coincidence, a typical overloading peak profile belonging to competitive Langmuir isotherm. The preparative separation was optimized by adjusting the sample size and detecting the sample at its low UV absorbance, and the positions of the cut points were determined by the detector response values. Even with an a value as small as 1.04, satisfactory preparative separations were observed by effectively utilized displacement effects and touching-band optimization. The recovery of epirubicin was 90% and the purity was more than 99% as measured by RPLC on a C-18 column. The added stepwise elution was a crucial procedure since it not only promoted the separation time but also regenerated the silica column to prolong its lifetime.
学科主题	Biochemistry & Molecular Biology ; Chemistry
WOS研究方向	Biochemistry & Molecular Biology ; Chemistry
WOS记录号	WOS:000233079500007
内容类型	期刊论文
源URL	[http://ir.rcees.ac.cn/handle/311016/23326]
专题	生态环境研究中心_水污染控制实验室
推荐引用方式 GB/T 7714	Li, RP,Huang, JX. Isolation and purification of epirubicin from raw product by preparative chromatography on a silica column with aqueous-rich mobile phase[J]. JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES,2005,28(17):2737-2751.
APA	Li, RP,&Huang, JX.(2005).Isolation and purification of epirubicin from raw product by preparative chromatography on a silica column with aqueous-rich mobile phase.JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES,28(17),2737-2751.
MLA	Li, RP,et al."Isolation and purification of epirubicin from raw product by preparative chromatography on a silica column with aqueous-rich mobile phase".JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES 28.17(2005):2737-2751.