Microbial DNA extraction and analyses of soil iron-manganese nodules

CORC > 生态环境研究中心 > 中国科学院生态环境研究中心 > 土壤环境科学实验室

	Microbial DNA extraction and analyses of soil iron-manganese nodules
	Feng, Xiong-Han; Zhang, Li-Mei; Liu, Fan; Tan, Wen-Feng; Zhu, Yong-Guan; He, Jizheng
刊名	SOIL BIOLOGY & BIOCHEMISTRY
	2008-06
卷号	40 期号:6 页码:1364-1369
关键词	Soil Fe-mn Nodules Dna Extraction 16s Rdna Ardra Mn(Ii)-oxidizing Bacteria
英文摘要	iron-manganese (Fe-Mn) nodules and concretions are soil new growth, reflecting soil environmental conditions during their formation. Bacteria play a dominant role in the oxidation of dissolved Mn(II) in aqueous systems and the formation of marine and freshwater Fe-Mn nodules. However, the role and significance of bacteria in soil Fe-Mn nodule formation have not been well recognized. In this paper, microbial DNA was directly extracted from two Fe-Mn nodule samples collected from Wuhan and Guiyang in central China. The extracted DNA was amplified by polymerase chain reaction (PCR) and cloned. The clones were then screened by amplified ribosomal DNA restriction analysis (ARDRA). Twenty patterns were obtained for Wuhan sample and Guiyang sample, respectively. DNA sequencing and phylogenetic analyses revealed that the bacterial compositions of the Fe-Mn nodules were mainly belonged to Firmicutes, beta-protcobacteria, gamma-proteobacteria branches of the domain bacteria. These divisions had close relativeness with Mn(II)-oxidizing bacteria identified from marine Fe-Mn nodules, implying the possible contributions of these bacteria to soil Fe-Mn nodule formation. (C) 2007 Elsevier Ltd. All rights reserved.
内容类型	期刊论文
源URL	[http://ir.rcees.ac.cn/handle/311016/22338]
专题	生态环境研究中心_土壤环境科学实验室
推荐引用方式 GB/T 7714	Feng, Xiong-Han,Zhang, Li-Mei,Liu, Fan,et al. Microbial DNA extraction and analyses of soil iron-manganese nodules[J]. SOIL BIOLOGY & BIOCHEMISTRY,2008,40(6):1364-1369.
APA	Feng, Xiong-Han,Zhang, Li-Mei,Liu, Fan,Tan, Wen-Feng,Zhu, Yong-Guan,&He, Jizheng.(2008).Microbial DNA extraction and analyses of soil iron-manganese nodules.SOIL BIOLOGY & BIOCHEMISTRY,40(6),1364-1369.
MLA	Feng, Xiong-Han,et al."Microbial DNA extraction and analyses of soil iron-manganese nodules".SOIL BIOLOGY & BIOCHEMISTRY 40.6(2008):1364-1369.