Arsenate-induced toxicity: Effects on antioxidative enzymes and DNA damage in Vicia faba

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	Arsenate-induced toxicity: Effects on antioxidative enzymes and DNA damage in Vicia faba
	Zhao, Fang-Jie; Lin, Aijun; Zhang, Xuhong; Zhu, Yong-Guan
刊名	ENVIRONMENTAL TOXICOLOGY AND CHEMISTRY
	2008-02
卷号	27 期号:2 页码:413-419
关键词	Arsenate Vicia Faba Oxidative Stress Dna Damage
英文摘要	A glasshouse hydroponic experiment was conducted to investigate the toxicity of arsenate to broad bean (Vicia-faba) plants when grown with external arsenate concentrations at 10 mu mol/L. The treated plants showed no obvious symptoms of phytotoxicity, but shoot/root growth was inhibited. Lipid peroxidation in leaves and roots increased with the addition of arsenate, indicating oxidative stress. We investigated the responses to arsenate exposure in the activities of several representative antioxidant enzymes, including peroxidase (POD), catalase (CAT), and superoxide dismutase (SOD), in plant tissues, and the DNA damage in plant leaves and root tips induced by arsenate in this plant was detected for the first time. Arsenate addition increased POD activity in the leaves significantly but decreased its activity in the roots in the 10 mu mol/L treatment. Arsenate addition caused an induction of SOD and CAT activities in both leaves and roots, but not in roots at the arsenate concentration of 10 mu mol/L. The DNA damage in V. faba was detected using Comet assay; in both leaves and roots, DNA damage increased with increasing arsenate concentrations, indicating genotoxicity of arsenate. These results indicate that arsenate toxicity causes oxidative stress in V. faba, which might be one of the mechanisms through which arsenic induces DNA damage.
WOS记录号	WOS:000252500000021
内容类型	期刊论文
源URL	[http://ir.rcees.ac.cn/handle/311016/22313]
专题	生态环境研究中心_土壤环境科学实验室
推荐引用方式 GB/T 7714	Zhao, Fang-Jie,Lin, Aijun,Zhang, Xuhong,et al. Arsenate-induced toxicity: Effects on antioxidative enzymes and DNA damage in Vicia faba[J]. ENVIRONMENTAL TOXICOLOGY AND CHEMISTRY,2008,27(2):413-419.
APA	Zhao, Fang-Jie,Lin, Aijun,Zhang, Xuhong,&Zhu, Yong-Guan.(2008).Arsenate-induced toxicity: Effects on antioxidative enzymes and DNA damage in Vicia faba.ENVIRONMENTAL TOXICOLOGY AND CHEMISTRY,27(2),413-419.
MLA	Zhao, Fang-Jie,et al."Arsenate-induced toxicity: Effects on antioxidative enzymes and DNA damage in Vicia faba".ENVIRONMENTAL TOXICOLOGY AND CHEMISTRY 27.2(2008):413-419.