Toxicity and transformation of graphene oxide and reduced graphene oxide in bacteria biofilm

CORC > 生态环境研究中心 > 中国科学院生态环境研究中心 > 环境化学与生态毒理学国家重点实验室

	Toxicity and transformation of graphene oxide and reduced graphene oxide in bacteria biofilm
	Zhao, Bin; Guo, Zhiling; Xie, Changjian; Zhang, Peng; Zhang, Junzhe; Wang, Guohua; He, Xiao; Ma, Yuhui; Zhang, Zhiyong
刊名	SCIENCE OF THE TOTAL ENVIRONMENT
	2017-09-01
卷号	580 期号:0 页码:1300-1308
关键词	Graphene Oxide Reduced Graphene Oxide Biofilm Toxicity Transformation
英文摘要	Impact of graphene based material (GNMs) on bacteria biofilm has not been well understood yet In this study, we compared the impact of graphene oxide (GO) and reduced graphene oxide (rGO) on biofilm formation and development in Luria-Bertani (LB) medium using Escherichia coli and Staphylococcus aureus as models. GO significantly enhanced the cell growth, biofilm formation, and biofilm development even up to a concentration of 500 mg/L. In contrast, rGO (>= 50 mg/L) strongly inhibited cell growth and biofilm formation. However, the inhibitory effects of rGO (50 mg/L and 100 mg/L) were attenuated in the mature phase (>24 h) and eliminated at 48 h. GO at 250 mg/L decreased the reactive oxygen species (ROS) levels in biofilm and extracellular region at mature phase. ROS levels were significantly increased by rGO at early phase, while they returned to the same levels as control at mature phase. These results suggest that oxidative stress contributed to the inhibitory effect of rGO on bacterial biofilm. We further found that supplement of extracellular polymeric substances (EPS) in the growth medium attenuated the inhibitory effect of rGO on the growth of developed biofilm. XPS results showed that rGO were oxidized to GO which can enhance the bacterial growth. We deduced that the elimination of the toxicity of rGO at mature phase was contributed by EPS protection and the oxidation of rGO. This study provides new insights into the interaction of GNMs with bacteria biofilm. (C) 2016 Elsevier B.V. All rights reserved.
内容类型	期刊论文
源URL	[http://ir.rcees.ac.cn/handle/311016/39444]
专题	生态环境研究中心_环境化学与生态毒理学国家重点实验室
推荐引用方式 GB/T 7714	Zhao, Bin,Guo, Zhiling,Xie, Changjian,et al. Toxicity and transformation of graphene oxide and reduced graphene oxide in bacteria biofilm[J]. SCIENCE OF THE TOTAL ENVIRONMENT,2017,580(0):1300-1308.
APA	Zhao, Bin.,Guo, Zhiling.,Xie, Changjian.,Zhang, Peng.,Zhang, Junzhe.,...&Zhang, Zhiyong.(2017).Toxicity and transformation of graphene oxide and reduced graphene oxide in bacteria biofilm.SCIENCE OF THE TOTAL ENVIRONMENT,580(0),1300-1308.
MLA	Zhao, Bin,et al."Toxicity and transformation of graphene oxide and reduced graphene oxide in bacteria biofilm".SCIENCE OF THE TOTAL ENVIRONMENT 580.0(2017):1300-1308.