Hematological Effects of Gold Nanorods on Erythrocytes: Hemolysis and Hemoglobin Conformational and Functional Changes

CORC > 生态环境研究中心 > 中国科学院生态环境研究中心 > 环境化学与生态毒理学国家重点实验室

	Hematological Effects of Gold Nanorods on Erythrocytes: Hemolysis and Hemoglobin Conformational and Functional Changes
	Lu, Dawei; Zhao, Xingchen; Liu, Qian S.; Li, Yiling; Feng, Rui; Hao, Fang; Qu, Guangbo; Zhou, Qunfang; Jiang, Guibin
刊名	ADVANCED SCIENCE
	2017-10-01
卷号	4 期号:12 页码:-
关键词	Bioconjugation Erythrocyte Gold Nanorods Hemoglobin Hemolysis
英文摘要	Gold nanorods (GNRs) are a unique class of metal nanostructures that have attractive potentials in biomedical applications, and the concern on their biological safety is concomitantly increasing. Hemocompatibility is extremely important as their contact with blood circulation is unavoidable during in vivo delivery. Herein, two kinds of GNRs coated with hexadecyltrimethylammonium bromide (C-GNRs) or poly(sodium-p-styrenesulfonate) are used to test their potential toxicological effects in blood. C-GNRs with positive surface charges efficiently induce hemolysis when encountering erythrocytes. Cellular internalization of C-GNRs is found, and they subsequently bind with hemoglobin, forming bioconjugates. The interaction between hemoglobin and C-GNR (stoichiometry 32.7: 1) is regulated by electrostatic forces. Chromophores like tryptophan (Trp) are found to interact with C-GNRs, causing enhancement in fluorescence intensity. The conformation of protein is partially altered, evidenced by decrease in alpha-helical, increase in beta-sheet and random coil of hemoglobin. Although C-GNRs do not essentially decrease oxygen binding capacity of hemoglobin, they hamper oxygen release from the protein. Heme, the oxygen binding unit, releases from hemoglobin upon C-GNR treatment, which could contribute to C-GNR-induced hemolysis. This study demonstrates the hematological effects of GNRs, revealing their potential risk in biomedical applications.
内容类型	期刊论文
源URL	[http://ir.rcees.ac.cn/handle/311016/39324]
专题	生态环境研究中心_环境化学与生态毒理学国家重点实验室
推荐引用方式 GB/T 7714	Lu, Dawei,Zhao, Xingchen,Liu, Qian S.,et al. Hematological Effects of Gold Nanorods on Erythrocytes: Hemolysis and Hemoglobin Conformational and Functional Changes[J]. ADVANCED SCIENCE,2017,4(12):-.
APA	Lu, Dawei.,Zhao, Xingchen.,Liu, Qian S..,Li, Yiling.,Feng, Rui.,...&Jiang, Guibin.(2017).Hematological Effects of Gold Nanorods on Erythrocytes: Hemolysis and Hemoglobin Conformational and Functional Changes.ADVANCED SCIENCE,4(12),-.
MLA	Lu, Dawei,et al."Hematological Effects of Gold Nanorods on Erythrocytes: Hemolysis and Hemoglobin Conformational and Functional Changes".ADVANCED SCIENCE 4.12(2017):-.