Deconvolution of single-cell multi-omics layers reveals regulatory heterogeneity

doi:10.1038/s41467-018-08205-7

	Deconvolution of single-cell multi-omics layers reveals regulatory heterogeneity
	Liu, Longqi 1,2,3; Liu, Chuanyu 1,2,4; Quintero, Andres 5,6; Wu, Liang 1,2,4; Yuan, Yue 1,2,4; Wang, Mingyue 1,2,4; Cheng, Mengnan 1,2,4; Leng, Lizhi 7,8; Xu, Liqin 1,2; Dong, Guoyi 1,2
刊名	NATURE COMMUNICATIONS
	2019-01-28
卷号	10 页码:10
ISSN号	2041-1723
DOI	10.1038/s41467-018-08205-7
通讯作者	Eils, Roland(roland.eils@bihealth.de) ; Shang, Zhouchun(shangzhouchun@genomics.cn) ; Xu, Xun(xuxun@genomics.cn)
英文摘要	Integrative analysis of multi-omics layers at single cell level is critical for accurate dissection of cell-to-cell variation within certain cell populations. Here we report scCAT-seq, a technique for simultaneously assaying chromatin accessibility and the transcriptome within the same single cell. We show that the combined single cell signatures enable accurate construction of regulatory relationships between cis-regulatory elements and the target genes at single-cell resolution, providing a new dimension of features that helps direct discovery of regulatory patterns specific to distinct cell identities. Moreover, we generate the first single cell integrated map of chromatin accessibility and transcriptome in early embryos and demonstrate the robustness of scCAT-seq in the precise dissection of master transcription factors in cells of distinct states. The ability to obtain these two layers of omics data will help provide more accurate definitions of "single cell state" and enable the deconvolution of regulatory heterogeneity from complex cell populations.
资助项目	Strategic Priority Research Program of the Chinese Academy of Sciences[XDA16010402] ; Shenzhen Municipal Government of China Peacock Plan[KQTD20150330171505310] ; Shenzhen Engineering Laboratory for Innovative Molecular Diagnostics[DRC-SZ (2016) 884] ; China Postdoctoral Science Foundation[2017M610553] ; NCT3.0/DKFZ grant within the ENHANCE project
WOS关键词	GENOME-WIDE DETECTION ; CHROMATIN ACCESSIBILITY ; NUCLEOTIDE ; EMBRYOS ; NUMBER
WOS研究方向	Science & Technology - Other Topics
语种	英语
出版者	NATURE PUBLISHING GROUP
WOS记录号	WOS:000456829100006
资助机构	Strategic Priority Research Program of the Chinese Academy of Sciences ; Shenzhen Municipal Government of China Peacock Plan ; Shenzhen Engineering Laboratory for Innovative Molecular Diagnostics ; China Postdoctoral Science Foundation ; NCT3.0/DKFZ grant within the ENHANCE project
内容类型	期刊论文
源URL	[http://119.78.100.205/handle/311034/9253]
专题	中国科学院古脊椎动物与古人类研究所
通讯作者	Eils, Roland; Shang, Zhouchun; Xu, Xun
作者单位	1.BGI Shenzhen, Shenzhen 518083, Peoples R China 2.BGI Shenzhen, China Natl GeneBank, Shenzhen 518120, Peoples R China 3.Xili Univ Town, Harbin Inst Technol, Shenzhen Grad Sch, Shenzhen 518055, Peoples R China 4.Univ Chinese Acad Sci, BGI Educ Ctr, Shenzhen 518083, Peoples R China 5.Div Theoret Bioinformat German Canc Res Ctr DKFZ, D-69120 Heidelberg, Germany 6.Univ Heidelberg Hosp, Hlth Data Sci Unit, D-69120 Heidelberg, Germany 7.Cent S Univ, Inst Reprod & Stem Cell Engn, Changsha 410078, Hunan, Peoples R China 8.Minist Hlth, Key Lab Stem Cells & Reprod Engn, Changsha 410078, Hunan, Peoples R China 9.Chinese Acad Sci, Inst Vertebrate Paleontol & Paleoanthropol, Beijing 100044, Peoples R China 10.Tongji Univ, Dept Regenerat Med, Sch Med, Shanghai 200092, Peoples R China
推荐引用方式 GB/T 7714	Liu, Longqi,Liu, Chuanyu,Quintero, Andres,et al. Deconvolution of single-cell multi-omics layers reveals regulatory heterogeneity[J]. NATURE COMMUNICATIONS,2019,10:10.
APA	Liu, Longqi.,Liu, Chuanyu.,Quintero, Andres.,Wu, Liang.,Yuan, Yue.,...&Xu, Xun.(2019).Deconvolution of single-cell multi-omics layers reveals regulatory heterogeneity.NATURE COMMUNICATIONS,10,10.
MLA	Liu, Longqi,et al."Deconvolution of single-cell multi-omics layers reveals regulatory heterogeneity".NATURE COMMUNICATIONS 10(2019):10.