QQ客服 官方微博 反馈留言

	Enrichment of Phosphopeptides by a Novel SAX Capillary Monolithic Column for Sensitive MALDI-TOF MS Analysis
	Dong MM(董铭铭) ; Wu MH(吴明火) ; Wang FJ(王方军) ; Qin HQ(秦洪强) ; Han GH(韩广辉) ; Dong J(董靖) ; Wu RA(吴仁安) ; Ye ML(叶明亮) ; Liu Z(刘震) ; Zou HF(邹汉法)
	2009-10-19
会议名称	the 24th international symposium on micro-scale bioseparation,
会议日期	2009-10-19
会议地点	中国
页码	259/1
通讯作者	邹汉法
中文摘要	protein phosphorylation is one of the most biologically relevant and ubiquitous post-translational modifications. maldi-tof ms is a powerful tool for the analysis of protein phosphorylation by detection of phosphopeptides in phosphoprotein digest. however, if protein digest is directly submitted to maldi-tof ms analysis, the sensitivity for detection of phosphopeptides is very poor due to the ion suppression by nonphosphorylated peptides. so the isolation and enrichment of phosphopeptides prior to ms analysis is crucial for protein phosphorylation analysis. here, a sax organic-silica hybrid capillary monolith was prepared to enrich phosphopeptides from protein digest for sensitive maldi-tof ms analysis. the sax monolithic column was prepared by a novel “one-pot” process by concurrently using organic monomers and alkoxysilanes. as the optimal loading and elution buffers for sax chromatography of phosphopeptides are basic and acidic solutions, respectively, the protein digests without any pretreatment could be loaded onto the sax column under basic ph conditions, after removing non-phosphopeptides by washing, the bound phosphopeptides were eluted with 5% formic acid directly onto a maldi target and analyzed by maldi-tof ms. above approach significantly reduced the sample loss and simplified the analytical procedures. because of the excellent ms detection compatible procedure and the nanoscale sax column, the detection limit of 50 amol for detection of phosphopeptides from β-casein digest was achieved.
会议主办者	大连化学物理研究所
学科主题	物理化学
语种	中文
内容类型	会议论文
源URL	[http://159.226.238.44/handle/321008/113840]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
推荐引用方式 GB/T 7714	Dong MM,Wu MH,Wang FJ,et al. Enrichment of Phosphopeptides by a Novel SAX Capillary Monolithic Column for Sensitive MALDI-TOF MS Analysis[C]. 见:the 24th international symposium on micro-scale bioseparation,. 中国. 2009-10-19.

个性服务

查看访问统计

相关权益政策

暂无数据

收藏/分享

除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。