| Spectroscopic Evidence of Human Telomeric G-quadruplex | |
| Zhou J(周俊) ; Feng ZC(冯兆池) ; Li C(李灿) | |
| 2009-09-13 | |
| 会议名称 | 第十三届亚洲化学会 |
| 会议日期 | 2009-9-13 |
| 会议地点 | china |
| 页码 | 77/1 |
| 通讯作者 | 李灿 |
| 中文摘要 | the structural interconversion between the g-quadruplex and duplex in vivo is an important subject because it is believed that the formation of g-quadruplex structure depends on the denaturation of the duplex [1]. in the present study, we used human telomeric dna duplex composed of ggg(ttaggg)3/ ccc(taaccc)3 as a model system to investigate its properties under near physiological conditions by spectroscopic methods. circular dichroism and fluorescence spectra demonstrated that g-quadruplex structure can be formed from duplex at near physiological ph (ph 7.4), salt concentration (150 mm k+), and temperature (37 oc) in the presence of molecular crowding agent peg (40 %), whereas the g-quadruplex structure can not be formed at 25 oc in buffer containing 150 mm k+ in the presence of peg. this work suggests that human telomeric g-quadruplex structure may be potentially formed from watson-crick duplex in vivo. |
| 会议主办者 | chinese chemical society |
| 学科主题 | 物理化学 |
| 语种 | 中文 |
| 内容类型 | 会议论文 |
| 源URL | [http://159.226.238.44/handle/321008/113816]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 推荐引用方式 GB/T 7714 | Zhou J,Feng ZC,Li C. Spectroscopic Evidence of Human Telomeric G-quadruplex[C]. 见:第十三届亚洲化学会. china. 2009-9-13. |
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