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	High sensitive protein detection with in-situ fluorescence derivatization
	Qiao XQ(乔晓强) ; Sun LL(孙良亮) ; Liang Z(梁振) ; Zhang LH(张丽华) ; Zhang YK(张玉奎)
	2009-10-19
会议名称	the 24th msb 2009
会议日期	2009-10-19
会议地点	中国
页码	242/1
通讯作者	张丽华
中文摘要	in the post-genome sequencing era, high sensitive protein analysis has been paid much attention. although native fluorescence emitted by aromatic amino acid residues is an effective tool for proteins detection, not all proteins could generate intensive native fluorescence signal. thus, various fluorescence reagents have been synthesized and applied for protein derivatization. however, the derivatization of low abundance proteins is still challenging. taking the traditional fluorescence reagent, fitc, as an example, the limit of detection for proteins is similar to that of uv detection. in current work, a novel in-situ fluorescence derivatization method based on hollow fiber membrane interface was developed to achieve high efficient derivatization and high sensitive detection of proteins, by which proteins were firstly concentrated in hollow fiber membrane, and then in situ derivatized by submerging the membrane into fluorescence buffer. with such a method, the high sensitive detection of proteins with pm concentration were achieved by hplc with a fluorescence detector. furthermore, the derivatization of a fraction of e. coli whole cell lysate were achieved, and high sensitive detection was obtained compared to that obtained by uv detection and conventional in-solution fluorescence derivatization.
会议主办者	大连化物所
学科主题	物理化学
语种	中文
内容类型	会议论文
源URL	[http://159.226.238.44/handle/321008/113614]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
推荐引用方式 GB/T 7714	Qiao XQ,Sun LL,Liang Z,et al. High sensitive protein detection with in-situ fluorescence derivatization[C]. 见:the 24th msb 2009. 中国. 2009-10-19.

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