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	Determination of paeonol O-demethylation activities in vitro by ultra-performance liquid chromatography with MS detection (UPLC-MS)
	Liu HX(刘慧鑫) ; Yang L(杨凌)
	2009-05-10
会议名称	3rd asian pacific regional meeting of the international society for the study of xenobiotics
会议日期	2009-5-10
会议地点	泰国
页码	63/1
通讯作者	yang ; l
中文摘要	o-demethylation of paeonol has been implicated as an important mechanism of paeonol elimination. to characterize paeonol metabolism by o-demethylation, a rapid and specific ultra-performance liquid chromatography-tandem mass spectrometry (uplc-ms) method was developed for the qualitative and quantitative determination of paeonol o-demethylation activity in human liver microsomes (hlms). hlms incubation system with paeonol resulted in the formation of a single product peak. following the purification, this peak was confirmed as o-demethylated metabolite of paeonol (2,4-dihydroxyacetophenone, dha) by ms and nmr. the method was validated for the determination of dha with respect to specificity, linearity, detection limit, recovery, stability, precision and accuracy. the chromatographic separation was achieved on a uplc beh c18 column (50 mm × 2.1 mm i.d., 1.7 μm), with phase of acetonitrile-water (ratio 30:70). selective ion reaction (sir) monitor was specific for paeonol, dha and i.s.. the method was linear over the concentration range 0.5-100 μm for dha in spiked hlms. acceptable precision and accuracy were obtained for concentrations over the standard curve range. dha were stable at 4°c for at least 72 h in spiked liver microsomes samples. the method was successfully used to determine the kinetics of dha activities toward paeonol in liver microsomes from different speices.
会议主办者	国际药物代谢学会
学科主题	物理化学
语种	中文
WOS记录号	WOS:000269483300123
内容类型	会议论文
源URL	[http://159.226.238.44/handle/321008/113566]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
推荐引用方式 GB/T 7714	Liu HX,Yang L. Determination of paeonol O-demethylation activities in vitro by ultra-performance liquid chromatography with MS detection (UPLC-MS)[C]. 见:3rd asian pacific regional meeting of the international society for the study of xenobiotics. 泰国. 2009-5-10.

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