| A NEW MULTINOZZLE ENCAPSULATION SYSTEM FOR THE LARGE-SCALE PRODUCTION OF UNIFORM ALGINATE BEADS | |
| Zhu J(朱静) ; Xie WY(谢威扬) ; Liu XD(刘袖洞) ; Zhang Y(张英) ; Yu WT(于炜婷) ; Wang W(王为) ; Ma XJ(马小军) | |
| 2008-10-12 | |
| 会议名称 | 13th international biotechnology symposium & exhibition |
| 会议日期 | 2008-10-12 |
| 会议地点 | 中国 |
| 页码 | s141/2 |
| 通讯作者 | 马小军 |
| 中文摘要 | abstract: purpose of study: over the past 20 years, cell microencapsulation technology has made a great progress in cell culture, artificial cell therapy, artificial organs in vitro and bioconversion. but, most of researches in preparation of microencapsulation based on the high voltage electrostatic technique are limited to the scope of the laboratory. to bring this technology into the clinic, it is great important to find a proper way for large scale preparation of biological microcapsule in a short time. therefore, we develop a new 19-nozzles system for the encapsulation of cells. methods: in this paper, calcium alginate gel beads were prepared through microcapsule preparation system under forced high-voltage electrostatic field developed in our lab designed with a 19-nozzle system for the encapsulation and immobilization of microorganisms, enzymes or cells. the effect of operation parameters on the large-scale preparation of gel beads was analyzed. the parameters included the applied potential (u) , the distribution of electrostatic field , the frequency and the flow rate of solution through the capillary. results and conclusions : the results show that the air pressure of feed is the decisive factor on the size and yield of gel beads, and the size distribution is dependent on several factors including the distribution of electric field, pulse potential, air pressure of feed, pulse frequency, pulse width and threshold potential etc. therefore, the monodisperse calcium alginate beads in the range of 0.2–4 mm were produced under sterile and reproducible conditions. the gel beads with good sphericity, smooth surface and low dispersivity (cv<20%) can be prepared by adjusting and matching above parameters. it is more important that the yield of gel beads by microcapsule preparation system is 10~60-fold higher than the traditional electrostatic droplet generator. |
| 会议主办者 | dalian institute of chemical physics |
| 学科主题 | 物理化学 |
| 语种 | 中文 |
| ISSN号 | 0168-1656 |
| WOS记录号 | WOS:000208979400323 |
| 内容类型 | 会议论文 |
| 源URL | [http://159.226.238.44/handle/321008/113424]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 推荐引用方式 GB/T 7714 | Zhu J,Xie WY,Liu XD,et al. A NEW MULTINOZZLE ENCAPSULATION SYSTEM FOR THE LARGE-SCALE PRODUCTION OF UNIFORM ALGINATE BEADS[C]. 见:13th international biotechnology symposium & exhibition. 中国. 2008-10-12. |
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