Electrosurgical knife is the most common invasive surgical instrument in a cutting and hemostasis process, which easily leads the overheated tissues to pyrolysis, eschar and adhesion on the knife surface. In order to minimize the adhesion of the overheated tissues, we investigated and fabricated the surface micro-structures on the blade by fiber laser inspired by shark's skin. The wettability of the blade surfaces with different bionic shark skins were measured by the contact angles, revealing the surface wettability effect. Frictional coefficients between the blade surfaces with different bionic shark skins and pork liver tissues are respectively measured by friction test machine. After cutting the pork liver, the adhesive tissue mass on the electrosurgical knives are weighed to study the antiadhesion. The relationship between adhesive mass, wettability and frictional coefficients on the bionic shark skins are analyzed in the orthogonal experimental method. The experimental results show that the bionic shark skin on the blades can effectively reduce the adhesive tissues. ? 2018 Japan Laser Processing Society.